Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 11094..11737 Replicon plasmid p1B33769
Accession NZ_CP072423
Organism Klebsiella pneumoniae strain B33769

Toxin (Protein)


Gene name vagD Uniprot ID A0A5Q9LNG2
Locus tag J6255_RS25600 Protein ID WP_048983057.1
Coordinates 11321..11737 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID R4IT22
Locus tag J6255_RS25595 Protein ID WP_016338373.1
Coordinates 11094..11324 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J6255_RS25580 (J6255_25575) 6835..7092 - 258 WP_023292103.1 hypothetical protein -
J6255_RS26515 7570..8298 + 729 Protein_6 CSS-motif domain-containing protein -
J6255_RS26520 8314..9150 + 837 WP_032439674.1 EAL domain-containing protein -
J6255_RS25590 (J6255_25585) 9768..10199 - 432 WP_174805835.1 hypothetical protein -
J6255_RS25595 (J6255_25590) 11094..11324 + 231 WP_016338373.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
J6255_RS25600 (J6255_25595) 11321..11737 + 417 WP_048983057.1 type II toxin-antitoxin system VapC family toxin Toxin
J6255_RS26525 11778..12101 - 324 WP_085842301.1 hypothetical protein -
J6255_RS26530 12119..12655 - 537 WP_223825639.1 hypothetical protein -
J6255_RS25610 (J6255_25605) 13314..14591 + 1278 WP_016338369.1 HlyD family secretion protein -
J6255_RS25615 (J6255_25610) 14581..16689 + 2109 WP_016338368.1 peptidase domain-containing ABC transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B / blaCTX-M-15 / dfrA14 / qnrB1 / tet(A) / aac(6')-Ib-cr / blaOXA-1 / aac(3)-IIa - 1..137142 137142


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-50)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15022.52 Da        Isoelectric Point: 9.2957

>T197765 WP_048983057.1 NZ_CP072423:11321-11737 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFARVPGLVLEDWVK

Download         Length: 417 bp

>T197765 NZ_CP096690:1191185-1191367 [Acinetobacter baumannii]
GTGAAAAGTCTGGATTTAATCAAAATGATTGAAGCAGACGGTTGGTATGAGGTTAGGGTTTCAGGAAGTCATCATCACTT
CAAACACCCAACCAAAAAGGGATTAGTAACAATCCCTCATCCTAAAAAGGATTTACCAAACGGAACTGTTAAAAGCATTT
TGAAGCAAGCGGGTCTAAATTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8751.77 Da        Isoelectric Point: 4.3533

>AT197765 WP_016338373.1 NZ_CP072423:11094-11324 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWSSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT197765 NZ_CP096690:1191432-1191836 [Acinetobacter baumannii]
ATGTTATATCCAATTGCTATTGAAAGAGGTACAGACACCGAAGCCTTTGGTGTCTCCGTTCCAGATATTCCAGGGTGTTT
CAGCGCAGGCGATACATTATATGAAGCTATCGAGAATGTTAAAGAGGCTATTTCTGGCCACTTGGAAATCCTAGCAGAAG
ATGGAGAGGAGATTCCTTTAGCATCTGATGTCAGTAAGTTTATTGACCAAGAAGATTATAGAGGTATGATCTGGGCAGTT
ACTGAAGTTGATGTTAGCCGTTACTTAGGTAAACCAGAAAAAATCAATGTTACTTTACCAAGCCGATTAATTCGGAAGAT
TGATGATAATGTTGGTAAAGATAAAAGATTTAAAACTCGCTCTGCTTTTTTGGCCGCTGGTGCTGAAAAGCTACTACATG
CTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5Q9LNG2

Antitoxin

Source ID Structure
AlphaFold DB A0A5Q9LN61

References