Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5231707..5232332 Replicon chromosome
Accession NZ_CP072422
Organism Klebsiella pneumoniae strain B33769

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag J6255_RS25190 Protein ID WP_002882817.1
Coordinates 5231707..5232090 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag J6255_RS25195 Protein ID WP_004150355.1
Coordinates 5232090..5232332 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J6255_RS25175 (J6255_25170) 5229073..5229975 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
J6255_RS25180 (J6255_25175) 5229972..5230607 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
J6255_RS25185 (J6255_25180) 5230604..5231533 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
J6255_RS25190 (J6255_25185) 5231707..5232090 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
J6255_RS25195 (J6255_25190) 5232090..5232332 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
J6255_RS25200 (J6255_25195) 5232537..5233454 + 918 WP_004895173.1 alpha/beta hydrolase -
J6255_RS25205 (J6255_25200) 5233468..5234409 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
J6255_RS25210 (J6255_25205) 5234454..5234891 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
J6255_RS25215 (J6255_25210) 5234888..5235748 - 861 WP_004146232.1 virulence factor BrkB family protein -
J6255_RS25220 (J6255_25215) 5235742..5236341 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T197764 WP_002882817.1 NZ_CP072422:c5232090-5231707 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T197764 NZ_CP096690:451006-451395 [Acinetobacter baumannii]
TTGATCAACCATTTGAATTTTAAGCTGAAATATAGTCGTTTTTCGATTATATTTCAGTTTTTTATTGGCTTAAGCTTAGC
AGTTCTATTTTATCAATTAATGCCGTTACTATGGTGGTTAGTTGTAATCAGCTTACTATTTATCAGCTTTATTTTCTTTT
TAAAGCGTCCGCAGCTTGCACAAATTGCTTATTTAGATAAAAAACTATGGTCGCTTGCTTATAAGTCACAGAACACCGTT
TCTCGCGTCAAAATTTTAAAAATAATTGATTGTCAGATTTTCATAGTGATCTATTTTGAAGGTCATAAAACACTAACTTC
TATCATTTGGTTTGATCAAATGTCATTAGCAGAGTGGAAAAAGCTTAAAACTTTAGAAAAACTCTATTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT197764 WP_004150355.1 NZ_CP072422:c5232332-5232090 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT197764 NZ_CP096690:450742-450999 [Acinetobacter baumannii]
ATGTCTGAAGAAATGACCTTGGAAGAACGTAAAGTAATCTATCGCGCACGTCGTGGTTTAAAGGAAATTGATGTCTATTT
TGACCCTTATGTGAAAAATTATTATTTAAAGGCAGACCCGGCAGAAAAAGCGTTGTTTGCTGAGTTGGTAGAACAGGAAG
ACCCTGATTTACTAGATTGGTTTATGGAAGTATCAGAACCACCACGTACCGAATTACGTGACTTTATCTATAAATTAAAA
CACTATGTTCATGGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References