Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5269135..5269760 Replicon chromosome
Accession NZ_CP072413
Organism Klebsiella pneumoniae strain B32278

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag J6256_RS25645 Protein ID WP_002882817.1
Coordinates 5269135..5269518 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag J6256_RS25650 Protein ID WP_004150355.1
Coordinates 5269518..5269760 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J6256_RS25630 (5266501) 5266501..5267403 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
J6256_RS25635 (5267400) 5267400..5268035 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
J6256_RS25640 (5268032) 5268032..5268961 + 930 WP_064162495.1 formate dehydrogenase accessory protein FdhE -
J6256_RS25645 (5269135) 5269135..5269518 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
J6256_RS25650 (5269518) 5269518..5269760 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
J6256_RS25655 (5269965) 5269965..5270882 + 918 WP_004181612.1 alpha/beta hydrolase -
J6256_RS25660 (5270896) 5270896..5271837 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
J6256_RS25665 (5271882) 5271882..5272319 - 438 WP_064162494.1 D-aminoacyl-tRNA deacylase -
J6256_RS25670 (5272316) 5272316..5273176 - 861 WP_004146232.1 virulence factor BrkB family protein -
J6256_RS25675 (5273170) 5273170..5273769 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T197746 WP_002882817.1 NZ_CP072413:c5269518-5269135 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T197746 NZ_CP096666:2342831-2343289 [Yersinia pestis EV76-CN]
ATGACGCTATACCGCATTGTTATGCGCCGCTATCTTGCATCGGCATGGACTGGTTACGGCGCTGAAATCTATGGTGGTCG
TTGGAATCATAAAGGCCATGCTGCCATCTATTTAGCCAGCAGTATTTCATTGGCTATGCTCGAAACACTGGCACATATTC
AGGACAGTTCAACACTCAGCGAATTTGAATTATTCCAAATTGAAATCAGTGACAGCAGTATTATGTTGCTACAGCCGCAG
GACTGGCCCACGGATTGGCGTAGCGATCCGGCACCGGTGGCAACGATGGATGTCGGCACCGAGTGGCTGGAATCAGAATC
CTCGGTAGGCTTACTGGTCCCGTCCACGTTGGTGCCGTCAGAAAACAACCTACTGGTCAATCCTCATCACAAAGATTTTC
CAGCGTGTTTGGCCAGCGTCAAACCGCTCTCTTTTGTCTTTGATCCACGGTTAAAATAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT197746 WP_004150355.1 NZ_CP072413:c5269760-5269518 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT197746 NZ_CP096666:2342323-2342769 [Yersinia pestis EV76-CN]
ATGAGAGCGTACCGCCCAAACCCCGCGACTAAAATGGGAACGCTTTGGCGTGAAATTGGCTTACCCGCCAGCCGCGGCAC
GATATTGGTCGATAGTATCAAAATGGGGTTTTCTGTTGATGTTATCGACAGCATTCATTTATGGGCATCCATGCCTAAAG
CTGAAATATTACGTGCTACCGGTATCCCCAGCCGTAGCCTGACTCGCCGCCGCACACATGATGGCCGTTTTACGCCTGAA
GAAAGCGAGAGAATCGCACGTTTTGTCCGCGTAATGGATGCCGCTGTCGATTTGTTCGGCGGCGATAAAGGTAAAGCCAT
TACCTGGATGTCCACGCCCATCAAAGGATTGGGGTACCGCAGCCCAGACTCCTTACTGGAAACCGAAACTGGCGCACTGG
AAGTGTGTGATTTGATTGGTCGTTTGGAACACGGCGTCTTTACTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References