Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4651779..4652589 Replicon chromosome
Accession NZ_CP072413
Organism Klebsiella pneumoniae strain B32278

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag J6256_RS22765 Protein ID WP_004178461.1
Coordinates 4651779..4652312 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag J6256_RS22770 Protein ID WP_002887278.1
Coordinates 4652323..4652589 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J6256_RS22760 (4650610) 4650610..4651731 + 1122 WP_004214138.1 cupin domain-containing protein -
J6256_RS22765 (4651779) 4651779..4652312 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
J6256_RS22770 (4652323) 4652323..4652589 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
J6256_RS22775 (4652692) 4652692..4654125 - 1434 WP_064162551.1 Cu(+)/Ag(+) sensor histidine kinase -
J6256_RS22780 (4654115) 4654115..4654798 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
J6256_RS22785 (4654970) 4654970..4656355 + 1386 WP_064162550.1 efflux transporter outer membrane subunit -
J6256_RS22790 (4656373) 4656373..4656717 + 345 WP_064162549.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T197744 WP_004178461.1 NZ_CP072413:c4652312-4651779 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T197744 NZ_CP096666:1825368-1825461 [Yersinia pestis EV76-CN]
TAAGCCTACATTAATACCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTAGACCGAATATCAGGAATCGTA
TTCGGTCTTTTTTT

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT197744 WP_002887278.1 NZ_CP072413:c4652589-4652323 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT197744 NZ_CP096666:c1825462-1825368 [Yersinia pestis EV76-CN]
TAAAAAAAGACCGAATACGATTCCTGATATTCGGTCTAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGT
ATTAATGTAGGCTTA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References