Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5277727..5278352 Replicon chromosome
Accession NZ_CP072408
Organism Klebsiella pneumoniae strain B4650

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag J6316_RS25705 Protein ID WP_002882817.1
Coordinates 5277727..5278110 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag J6316_RS25710 Protein ID WP_004150355.1
Coordinates 5278110..5278352 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J6316_RS25690 (5275093) 5275093..5275995 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
J6316_RS25695 (5275992) 5275992..5276627 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
J6316_RS25700 (5276624) 5276624..5277553 + 930 WP_064162495.1 formate dehydrogenase accessory protein FdhE -
J6316_RS25705 (5277727) 5277727..5278110 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
J6316_RS25710 (5278110) 5278110..5278352 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
J6316_RS25715 (5278557) 5278557..5279474 + 918 WP_004181612.1 alpha/beta hydrolase -
J6316_RS25720 (5279488) 5279488..5280429 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
J6316_RS25725 (5280474) 5280474..5280911 - 438 WP_064162494.1 D-aminoacyl-tRNA deacylase -
J6316_RS25730 (5280908) 5280908..5281768 - 861 WP_004146232.1 virulence factor BrkB family protein -
J6316_RS25735 (5281762) 5281762..5282361 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T197730 WP_002882817.1 NZ_CP072408:c5278110-5277727 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T197730 NZ_CP096664:797104-797451 [Pseudomonas aeruginosa]
ATGTCCCCGGTCGTCATTCGTTTTACTGATACCGCAGAGCAAAGCATCGAAGACCAAGTCCACCACTTGGCTCCATTCCA
AGGTGAACAGGCTGCACTCCAGTCAGTACTGAGCCTTTTGGATGAGATTGAAGAGAAGATTTCACTTGCACCTAAAGGTT
ACCCAGTCAGCCAGCAGGCGAGTCTTCTGGGGGTGCTGAGCTATCGCGAGCTTAATACCGGCCCCTATCGTGTTTTTTAC
GAATTCCACGAAGAGCAAGGCGAGGTGGCAGTGATCTTGGTTTTGCGACAGAAGCAGAGCGTTGAGCAGCAATTGATCCG
CTACTGCTTGGTGGGGCCAATCGAGTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT197730 WP_004150355.1 NZ_CP072408:c5278352-5278110 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT197730 NZ_CP096664:796843-797094 [Pseudomonas aeruginosa]
ATGCGAGTCGAGACAATTAGTTATTTGAAACGTCATGCGGCTGACCTGGATTTATCCGAGCCAATGGTCGTCACGCAGAA
CGGTGTTCCTGCCTATGTGGTTGAGTCATATGCTGAGCGGAAGCAGCGCGATGAAGCAATTGCGCTGGTGAAGTTGCTTG
CGATTGGCTCCCGCCAGTACGCAGAAGGCAAGCATCGCTCTGTTGATGATTTGAAAGCTCGCCTTTCCAGGAGGTTCGCT
CAGCCAGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References