Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4660371..4661181 Replicon chromosome
Accession NZ_CP072408
Organism Klebsiella pneumoniae strain B4650

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag J6316_RS22825 Protein ID WP_004178461.1
Coordinates 4660371..4660904 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag J6316_RS22830 Protein ID WP_002887278.1
Coordinates 4660915..4661181 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J6316_RS22820 (4659202) 4659202..4660323 + 1122 WP_004214138.1 cupin domain-containing protein -
J6316_RS22825 (4660371) 4660371..4660904 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
J6316_RS22830 (4660915) 4660915..4661181 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
J6316_RS22835 (4661284) 4661284..4662717 - 1434 WP_064162551.1 Cu(+)/Ag(+) sensor histidine kinase -
J6316_RS22840 (4662707) 4662707..4663390 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
J6316_RS22845 (4663562) 4663562..4664947 + 1386 WP_064162550.1 efflux transporter outer membrane subunit -
J6316_RS22850 (4664965) 4664965..4665309 + 345 WP_064162549.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T197728 WP_004178461.1 NZ_CP072408:c4660904-4660371 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T197728 NZ_CP096649:75194-75469 [Fenollaria massiliensis]
ATGAAATATAAGGTCTTATTAACATCTAAATTTAAAAAAGACTTTAAATTATTAAAAAAGCAGGGCAAGAATACAGATAA
AATTTTAGCCATTATTAATAAGCTAGCTAAAGGCGAATTTTTAGATGCAAAATACAAGGATCATCCTTTGTTAGGAGATT
ATCTCGGCGCAAGAGAGTGCCATATTGAACCAGATTTACTTCTAATATATGAAAAACAAGAAAATATTCTGATACTTAAT
ATATTGAGAGCTGGTTCACATTCAAATCTATTTTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT197728 WP_002887278.1 NZ_CP072408:c4661181-4660915 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT197728 NZ_CP096649:74925-75197 [Fenollaria massiliensis]
ATGAGTAACACTAACTTAAATGTAAGGATAGATAAGGACATAAAAAAGGCTGCGGAAGAAGTTTACGCTGAACTTGGCTT
TAATATGTCGACAGCCATAAATATGTTTTTAAGAGCAAGCATCAGAAAAGGTGGCATTCCTTTTGATTTAAAACTAGATG
TGCCTAATAAAGAGACTTTAGAAGCGATTGAAGAAGGAAGACGCTTAGCAATGGATCCTTCAACACCGCTATATGACAAT
ATGGAAGATCTTAGAAGAGCTTTAGAAGTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References