Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4056880..4057499 Replicon chromosome
Accession NZ_CP072408
Organism Klebsiella pneumoniae strain B4650

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag J6316_RS19910 Protein ID WP_002892050.1
Coordinates 4057281..4057499 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag J6316_RS19905 Protein ID WP_002892066.1
Coordinates 4056880..4057254 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J6316_RS19895 (4052032) 4052032..4053225 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
J6316_RS19900 (4053248) 4053248..4056394 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
J6316_RS19905 (4056880) 4056880..4057254 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
J6316_RS19910 (4057281) 4057281..4057499 + 219 WP_002892050.1 HHA domain-containing protein Toxin
J6316_RS19915 (4057662) 4057662..4058228 + 567 WP_002892030.1 maltose O-acetyltransferase -
J6316_RS19920 (4058200) 4058200..4058340 - 141 WP_004147370.1 hypothetical protein -
J6316_RS19925 (4058361) 4058361..4058831 + 471 WP_002892026.1 YlaC family protein -
J6316_RS19930 (4058806) 4058806..4060257 - 1452 WP_064162152.1 PLP-dependent aminotransferase family protein -
J6316_RS19935 (4060358) 4060358..4061056 + 699 WP_004177238.1 GNAT family protein -
J6316_RS19940 (4061053) 4061053..4061193 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
J6316_RS19945 (4061193) 4061193..4061456 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T197727 WP_002892050.1 NZ_CP072408:4057281-4057499 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T197727 NZ_CP096596:c4133276-4132986 [Gordonia amicalis]
ATGAGCGACGCTCCGACAGACTCGCCCGCCCCGTACCGGGTCGAGGTCGCATCCCCAGCTCGCCGCGACCTTCAACGCCT
TCCATCCCGGATCGTCCACGCCGTCATCGAATTCATCTCCGGACCTCTCGCTGGTAACCCACACCGGCTCAGCAAGCCGC
TGCGCAACGACCTCGCCGGCCTCCACAGCGCCCGACGCGGCGACTACCGCGTCCTGCTCCGCATCGACGACCCGAACCAC
ACCATCGTTGTCGTCAGAATCGACCACCGTGCCCACGCCTATCGCACCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT197727 WP_002892066.1 NZ_CP072408:4056880-4057254 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT197727 NZ_CP096596:c4133548-4133273 [Gordonia amicalis]
ATGACGACAGTGCCACTGGGTGAAGCCAAGGACAAGCTCTCCGCGCTGGTCGACAGCGCCGAAACCACGCACGACATCAT
CACGATCACCAAGCACGGCAAACCCGCCGCGGTACTGATGTCGGCCGACGATCTCGAATCGCTGCACGAGACGATCTACT
GGCTCTCACGTGCCGGTGTCCGCGACGACGTCGCTACCGCCGACGCCGAGTACGCCGCCGGCCACACCGTCTCCATCGAC
GACCTGCGCGCCGAACACGGCATGCCGCCGAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References