Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5277885..5278510 Replicon chromosome
Accession NZ_CP072403
Organism Klebsiella pneumoniae strain B13243

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag J6322_RS25710 Protein ID WP_002882817.1
Coordinates 5277885..5278268 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag J6322_RS25715 Protein ID WP_004150355.1
Coordinates 5278268..5278510 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J6322_RS25695 (5275251) 5275251..5276153 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
J6322_RS25700 (5276150) 5276150..5276785 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
J6322_RS25705 (5276782) 5276782..5277711 + 930 WP_064162495.1 formate dehydrogenase accessory protein FdhE -
J6322_RS25710 (5277885) 5277885..5278268 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
J6322_RS25715 (5278268) 5278268..5278510 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
J6322_RS25720 (5278715) 5278715..5279632 + 918 WP_004181612.1 alpha/beta hydrolase -
J6322_RS25725 (5279646) 5279646..5280587 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
J6322_RS25730 (5280632) 5280632..5281069 - 438 WP_064162494.1 D-aminoacyl-tRNA deacylase -
J6322_RS25735 (5281066) 5281066..5281926 - 861 WP_004146232.1 virulence factor BrkB family protein -
J6322_RS25740 (5281920) 5281920..5282519 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T197714 WP_002882817.1 NZ_CP072403:c5278268-5277885 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T197714 NZ_CP096589:112428-112826 [Escherichia coli]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTTACGATAAAGAACAAACCCGCCAGTGTCAGGGAACGTTTTAA
CCTGAACCAGGGGAGAATGTGCATCAGTTCGGTCACTCTGATGGAGGTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGACGTTCTGGATTACGACGCTGCTGCTGCCACACACACC
GGCCAGATAAGAGCAGAACTTGCCCTTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGACTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGGCGGCCTGAGAATTGAAGACTGGAGTTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT197714 WP_004150355.1 NZ_CP072403:c5278510-5278268 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT197714 NZ_CP096589:112201-112428 [Escherichia coli]
ATGGAAACCACCGTATTTCTCAGCAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCCGAATCATTACGCCTGCCGGAGAGACATGGGACGAATGGTTCGACGGAA
ACAGTGTCAGCGCTGATTTTATGGATAACAGGGAACAGCCTGGTATGCAGGAAAGGGAGTCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References