Detailed information of TA system TA197709

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2677120..2678708
• Replicon: chromosome
• Accession: NZ_CP072403
• Organism: Klebsiella pneumoniae strain B13243

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: J6322_RS13095
• Protein ID: WP_064162061.1
• Coordinates: 2677120..2678442 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A0H3GXD8
• Locus tag: J6322_RS13100
• Protein ID: WP_002904790.1
• Coordinates: 2678442..2678708 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J6322_RS13080 (2672176)	2672176..2674677	+	2502	WP_064162059.1	fimbria/pilus outer membrane usher protein	-
J6322_RS13085 (2674665)	2674665..2675669	+	1005	WP_064162060.1	fimbrial protein	-
J6322_RS13090 (2675811)	2675811..2677103	+	1293	WP_002904794.1	glycoside hydrolase family 10 protein	-
J6322_RS13095 (2677120)	2677120..2678442	-	1323	WP_064162061.1	type II toxin-antitoxin system HipA family toxin	Toxin
J6322_RS13100 (2678442)	2678442..2678708	-	267	WP_002904790.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
J6322_RS13105 (2678974)	2678974..2679300	-	327	WP_064162062.1	hydroxyisourate hydrolase	-
J6322_RS13110 (2679297)	2679297..2679797	-	501	WP_064162063.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
J6322_RS13115 (2679775)	2679775..2681154	-	1380	WP_040245186.1	nucleobase:cation symporter-2 family protein	-
J6322_RS13120 (2681500)	2681500..2682654	+	1155	WP_002904783.1	FAD-dependent urate hydroxylase HpxO	-
J6322_RS13125 (2682635)	2682635..2683558	-	924	WP_002904781.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49155.46 Da
• Isoelectric Point: 7.5357

>T197709 WP_064162061.1 NZ_CP072403:c2678442-2677120 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITSQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIIATPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSTHVWQAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T197709 NZ_CP096587:3764884-3765162 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
ATTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9906.27 Da
• Isoelectric Point: 8.5912

>AT197709 WP_002904790.1 NZ_CP072403:c2678708-2678442 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKAAETAPAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT197709 NZ_CP096587:3764621-3764881 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GXD8