Detailed information of TA system TA197677

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1291184..1291758
• Replicon: chromosome
• Accession: NZ_CP072385
• Organism: Arachnia propionica strain F0714

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A4P8ZSL9
• Locus tag: J5A53_RS05960
• Protein ID: WP_014846982.1
• Coordinates: 1291411..1291758 (+)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: J5A53_RS05955
• Protein ID: WP_095532222.1
• Coordinates: 1291184..1291411 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J5A53_RS05940 (J5A53_05940)	1287095..1287424	+	330	WP_123824199.1	hypothetical protein	-
J5A53_RS05945 (J5A53_05945)	1287623..1288159	+	537	WP_014846985.1	transcriptional regulator NrdR	-
J5A53_RS05950 (J5A53_05950)	1288197..1291046	+	2850	WP_014846984.1	vitamin B12-dependent ribonucleotide reductase	-
J5A53_RS05955 (J5A53_05955)	1291184..1291411	+	228	WP_095532222.1	ribbon-helix-helix domain-containing protein	Antitoxin
J5A53_RS05960 (J5A53_05960)	1291411..1291758	+	348	WP_014846982.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
J5A53_RS15625	1292361..1292589	-	229	Protein_1172	ISL3 family transposase	-
J5A53_RS05965 (J5A53_05965)	1292824..1294854	-	2031	WP_123824201.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-115)

Antitoxin

• Ribbon-helix-helix protein, CopG (6-40)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 12461.49 Da
• Isoelectric Point: 11.7369

>T197677 WP_014846982.1 NZ_CP072385:1291411-1291758 [Arachnia propionica]
VLRGEIRLVDLEPARAGEADKRRPAIIVSNNRANTVAARLGRGVVTVVPVTSNTARVFPFQVLLTADEVGIRLDSKAQAE
QVRAVSVDRIGPVIGRLPARLVAQLDEALRLHLQL

Nucleotide

• Length: 348 bp

>T197677 NZ_CP096570:4234661-4234912 [Xanthomonas translucens pv. undulosa]
ATGGACACCATCACCTATAGCGCTGCACGCGCCGCCCTTGCGGACACCATGGAGCGCGTCGTCAACAACCACGAGCCGGT
GATCATCACCCGCAGCCGCGAGCAATCCGTGGTCATGCTGTCGCTTGAAGACTACAAGGCGATGGAGGAAACCGCCTACC
TGCTGCGCAGTCCGAAGAGCGCGCAGCGCCTGCTGGAATCCATCGCCCAGCTCGAGGCCGGTCGCGGCAAGGCACGGGAC
CTGGCCGAGTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 7907.80 Da
• Isoelectric Point: 4.0390

>AT197677 WP_095532222.1 NZ_CP072385:1291184-1291411 [Arachnia propionica]
MGQVKLSVSLSPSEVAVLDKYAQSAGLKSRSAAIQQAIKLLGDPDLEDAYTAAWQEWEDAGEAEAWAATVADGVK

Nucleotide

• Length: 228 bp

>AT197677 NZ_CP096570:4234909-4235166 [Xanthomonas translucens pv. undulosa]
GTGACGCTTCAGTTCTCGGACAATGCCTGGGAGGACGACCTCTACTGGCAGCAAACCGACAAGAAGATGCTCAAGCGCGT
CAACGATCTGATCAAAGCCATTCAACGCGATCCCTTCCAAGGCGTCGGCAAGCCAGAACCGCTGCGCCATGCCTTGGCCG
GCTACTGGTCGCGGCGCATCAACGAAGAACACCGCATCGTGTACAGGGTCGAGGCGGGCATCCTGCTGATCGCGCAGGTG
CGCTACCACTATCTGTAG

Structures

Toxin

• AlphaFold DB: A0A4P8ZSL9