Detailed information of TA system TA197648

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 33301..33848
• Replicon: plasmid unnamed1
• Accession: NZ_CP072323
• Organism: Escherichia coli strain EC-JS426

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: J6247_RS23160
• Protein ID: WP_244449879.1
• Coordinates: 33301..33579 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A075M9N8
• Locus tag: J6247_RS23165
• Protein ID: WP_032072813.1
• Coordinates: 33579..33848 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J6247_RS24525 (30782)	30782..31082	+	301	Protein_23	hypothetical protein	-
J6247_RS23145 (31160)	31160..31507	-	348	WP_000142437.1	DUF6404 family protein	-
J6247_RS23150 (31809)	31809..32290	-	482	Protein_25	hypothetical protein	-
J6247_RS23155 (32407)	32407..33255	-	849	WP_054444119.1	3'-5' exonuclease	-
J6247_RS23160 (33301)	33301..33579	-	279	WP_244449879.1	type II toxin-antitoxin system toxin YacB	Toxin
J6247_RS23165 (33579)	33579..33848	-	270	WP_032072813.1	type II toxin-antitoxin system antitoxin YacA	Antitoxin
J6247_RS23170 (34763)	34763..35620	-	858	WP_000130940.1	incFII family plasmid replication initiator RepA	-
J6247_RS24530 (35613)	35613..35660	-	48	WP_077822033.1	hypothetical protein	-
J6247_RS24535 (35651)	35651..35887	+	237	WP_223368648.1	replication protein RepA	-
J6247_RS23180 (35913)	35913..36173	-	261	WP_000083818.1	replication regulatory protein RepA	-
J6247_RS23185 (36412)	36412..36726	-	315	Protein_33	DUF2726 domain-containing protein	-
J6247_RS23190 (36759)	36759..38510	-	1752	Protein_34	Tn3-like element TnAs1 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / floR / aph(6)-Id / aph(3'')-Ib / aph(3')-Ia / ant(3'')-Ia / sul3 / aadA2 / blaTEM-1B / mph(A) / dfrA14 / sitABCD	iroB / iroC / iroD / iroE / iroN	1..142200	142200
	-	flank	IS/Tn	-	-	29919..30422	503

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10817.45 Da
• Isoelectric Point: 8.4615

>T197648 WP_244449879.1 NZ_CP072323:c33579-33301 [Escherichia coli]
MEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASNLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVY
ILRVLHTVQKWP

Nucleotide

• Length: 279 bp

>T197648 NZ_CP096530:1626213-1626674 [Staphylococcus aureus]
ATGGGATTATATGAAGAAACTTTAATACAACATGATTATATTGAAATAAGAGAGGCTGATGTGCTTCCAGATAATTTGGA
TGGGGTATGGTTAGGAGATTTAATTTTAATAAAGCGTGGCTTATCAGATAGAGAAAAGGCAGGAATTCTCTTTGAAGAAT
TAGCACATAATAAACTTACATACGGTGATATAGCCGATTACTCGAAATTCAACAATCGCAAGTTCGAAAATTACGCAAGG
CGACACGGCTTTATCTCAGCAGTCCCGTTACGCGAAATTGTGGAAGCTTACAATTATGGTGTACGTAACTTGTATGAGTT
GTCTGAGTATCTGCAATTGAGTGAAGAATACATATTAGAAGCAATAGAACAATATAAAAAGATATATGGTATTGGGACTC
ACTACGGCGAATACTCAATTACATTTGAGCCATTGAGAGTTTTTAAATATAAAGAAATATAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10277.64 Da
• Isoelectric Point: 10.2879

>AT197648 WP_032072813.1 NZ_CP072323:c33848-33579 [Escherichia coli]
MAQVNMNLRINAEIKNAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT197648 NZ_CP096530:1625886-1626200 [Staphylococcus aureus]
ATGTGCTTTTCAAAAAGAATGAAACAATCAAGAGAAAAACAAGGTATGACTTTGGCCGAACTAGGAAGAAAAATTGGTAA
AACTGAAGCTACTGTACAGCGTTATGAAAGCGGAAATATCAAAAATTTAAAAAACGATACTATAGAAAGTATAGCTACTG
CATTAAATGTTAATCCTGCATATTTAATGGGGTGGGTTGAAGAAAACGATGATGAAGTACAACATCGTGCAGCTCACCTT
GAAGGAGAATTGACAGATGATGAATGGCAAAGAGTTTTAGATTATGCAGATTATATAAGAAGCAAACGTAAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A075M9N8