Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 4425083..4425708 Replicon chromosome
Accession NZ_CP072322
Organism Escherichia coli strain EC-JS426

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag J6247_RS21170 Protein ID WP_000911329.1
Coordinates 4425310..4425708 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag J6247_RS21165 Protein ID WP_000450524.1
Coordinates 4425083..4425310 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J6247_RS21140 (4420884) 4420884..4421354 - 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -
J6247_RS21145 (4421354) 4421354..4421926 - 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
J6247_RS21150 (4422072) 4422072..4422950 + 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
J6247_RS21155 (4422967) 4422967..4424001 + 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
J6247_RS21160 (4424214) 4424214..4424927 + 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
J6247_RS21165 (4425083) 4425083..4425310 + 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
J6247_RS21170 (4425310) 4425310..4425708 + 399 WP_000911329.1 type II toxin-antitoxin system VapC family toxin Toxin
J6247_RS21175 (4425855) 4425855..4426718 + 864 WP_001267507.1 neutral zinc metallopeptidase -
J6247_RS21180 (4426733) 4426733..4428748 + 2016 WP_000829332.1 tRNA cytosine(34) acetyltransferase TmcA -
J6247_RS21185 (4428822) 4428822..4429520 + 699 WP_000679812.1 esterase -
J6247_RS21190 (4429630) 4429630..4429830 - 201 WP_000383836.1 YpfN family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T197647 WP_000911329.1 NZ_CP072322:4425310-4425708 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T197647 NZ_CP096530:c904844-904656 [Staphylococcus aureus]
ATGTCTTTACATTTTGCAATTCTGTTTTGGCTAGCATTAATTTTCTTAGTTGCCGCTACGTTTATACTCGTATTAATGAA
AAAAACTGGCAAAGAATCTAAAAAAGAGTCCTATTTAAGTTTCACTGTCATTCTCTATATTTTTGGATTCGCTATATTAA
TATACACATTTATATTTGGTGTGCTATAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT197647 WP_000450524.1 NZ_CP072322:4425083-4425310 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT197647 NZ_CP096530:c905132-904872 [Staphylococcus aureus]
ATGAATAACATTTTGTTAAATGCTATCAATATAGTTATTACTACCACTTTTGTTATCTTTAATATTTTAATCACATATAA
TAAAGATTTAGATGATTTATGTTGGCTCCTGCCTGGTATTATCATTTGTGGTGTGATACTCATAGTATCCTTTACCATTG
CAATGATAACTAAAAACTGGTTAAGTGAAATATTATTTTTTATAAATATCGTACTCGTTCTCTATTACATTTATCCTATT
TTTTATAGTTTTATAGGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References