Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 4142316..4142899 Replicon chromosome
Accession NZ_CP072322
Organism Escherichia coli strain EC-JS426

Toxin (Protein)


Gene name mazF Uniprot ID U9XFN8
Locus tag J6247_RS19785 Protein ID WP_000254745.1
Coordinates 4142564..4142899 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag J6247_RS19780 Protein ID WP_000581937.1
Coordinates 4142316..4142564 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J6247_RS19770 (4138655) 4138655..4139956 + 1302 WP_000046790.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
J6247_RS19775 (4140004) 4140004..4142238 + 2235 WP_027662018.1 GTP diphosphokinase -
J6247_RS19780 (4142316) 4142316..4142564 + 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
J6247_RS19785 (4142564) 4142564..4142899 + 336 WP_000254745.1 endoribonuclease MazF Toxin
J6247_RS19790 (4142970) 4142970..4143761 + 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
J6247_RS19795 (4143989) 4143989..4145626 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
J6247_RS19800 (4145714) 4145714..4147012 + 1299 WP_000036723.1 phosphopyruvate hydratase -
J6247_RS19805 (4147068) 4147068..4147430 - 363 WP_000034928.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12158.08 Da        Isoelectric Point: 8.4777

>T197646 WP_000254745.1 NZ_CP072322:4142564-4142899 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T197646 NZ_CP096528:c2130019-2129810 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATTGATGGTCAAGTAACGGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATTTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT197646 WP_000581937.1 NZ_CP072322:4142316-4142564 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT197646 NZ_CP096528:c2130324-2130031 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGAGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYM3

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References