Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3968257..3968911 Replicon chromosome
Accession NZ_CP072322
Organism Escherichia coli strain EC-JS426

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag J6247_RS19000 Protein ID WP_000244772.1
Coordinates 3968504..3968911 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag J6247_RS18995 Protein ID WP_000354046.1
Coordinates 3968257..3968523 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J6247_RS18975 (3964544) 3964544..3964855 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
J6247_RS18980 (3965019) 3965019..3965678 + 660 WP_000250274.1 hemolysin III family protein -
J6247_RS18985 (3965801) 3965801..3966781 - 981 WP_000399648.1 IS110-like element IS621 family transposase -
J6247_RS18990 (3967034) 3967034..3968014 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
J6247_RS18995 (3968257) 3968257..3968523 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
J6247_RS19000 (3968504) 3968504..3968911 + 408 WP_000244772.1 protein YgfX Toxin
J6247_RS19005 (3968951) 3968951..3969472 - 522 WP_001055874.1 flavodoxin FldB -
J6247_RS19010 (3969584) 3969584..3970480 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
J6247_RS19015 (3970505) 3970505..3971215 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
J6247_RS19020 (3971221) 3971221..3972954 + 1734 WP_000813220.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 3965801..3966781 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T197645 WP_000244772.1 NZ_CP072322:3968504-3968911 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T197645 NZ_CP096528:c2110435-2110073 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAACAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAGCAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTAAATGCAGTAGCTCATCAGAAAAATTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT197645 WP_000354046.1 NZ_CP072322:3968257-3968523 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT197645 NZ_CP096528:c2110602-2110432 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References