Detailed information of TA system TA197638

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 1845392..1846229
• Replicon: chromosome
• Accession: NZ_CP072322
• Organism: Escherichia coli strain EC-JS426

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: J6247_RS08970
• Protein ID: WP_000227784.1
• Coordinates: 1845687..1846229 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: J6247_RS08965
• Protein ID: WP_001297137.1
• Coordinates: 1845392..1845703 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J6247_RS08940 (1840412)	1840412..1841359	+	948	WP_001239440.1	cytochrome o ubiquinol oxidase subunit II	-
J6247_RS08945 (1841381)	1841381..1843372	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
J6247_RS08950 (1843362)	1843362..1843976	+	615	WP_000179814.1	cytochrome o ubiquinol oxidase subunit III	-
J6247_RS08955 (1843976)	1843976..1844305	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
J6247_RS08960 (1844317)	1844317..1845207	+	891	WP_000971336.1	heme o synthase	-
J6247_RS08965 (1845392)	1845392..1845703	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
J6247_RS08970 (1845687)	1845687..1846229	+	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
J6247_RS08975 (1846285)	1846285..1847220	-	936	WP_001297127.1	tetratricopeptide repeat protein	-
J6247_RS08980 (1847628)	1847628..1848992	+	1365	WP_001000978.1	MFS transporter	-
J6247_RS08985 (1849120)	1849120..1849611	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
J6247_RS08990 (1849779)	1849779..1850690	+	912	WP_000705874.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T197638 WP_000227784.1 NZ_CP072322:1845687-1846229 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T197638 NZ_CP096526:c345494-345033 [Staphylococcus aureus]
ATGGGATTATATGAAGAAACTTTAATACAACATGATTATATTGAAATAAGAGAGGCTGATGTGCTTCCAGATAATTTGGA
TGGGGTATGGTTAGGAGATTTAATTTTAATAAAGCGTGGCTTATCAGATAGAGAAAAGGCAGGAATTCTCTTTGAAGAAT
TAGCACATAATAAACTTACATACGGTGATATAGCCGATTACTCGAAATTCAACAATCGCAAGTTCGAAAATTACGCAAGG
CGACACGGCTTTATCTCAGCAGTCCCGTTACGCGAAATTGTGGAAGCTTACAATTATGGTGTACGTAACTTGTATGAGTT
GTCTGAGTATCTGCAATTGAGTGAAGAATACATATTAGAAGCAATAGAACAATATAAAAAGATATATGGTATTGGGACTC
ACTACGGCGAATACTCAATTACATTTGAGCCATTGAGAGTTTTTAAATATAAAGAAATATAA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT197638 WP_001297137.1 NZ_CP072322:1845392-1845703 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT197638 NZ_CP096526:c345821-345507 [Staphylococcus aureus]
ATGTGCTTTTCAAAAAGAATGAAACAATCAAGAGAAAAACAAGGTATGACTTTGGCCGAACTAGGAAGAAAAATTGGTAA
AACTGAAGCTACTGTACAGCGTTATGAAAGCGGAAATATCAAAAATTTAAAAAACGATACTATAGAAAGTATAGCTACTG
CATTAAATGTTAATCCTGCATATTTAATGGGGTGGGTTGAAGAAAACGATGATGAAGTACAACATCGTGCAGCTCACCTT
GAAGGAGAATTGACAGATGATGAATGGCAAAGAGTTTTAGATTATGCAGATTATATAAGAAGCAAACGTAAGTAA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3