Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1811313..1811931 Replicon chromosome
Accession NZ_CP072322
Organism Escherichia coli strain EC-JS426

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag J6247_RS08800 Protein ID WP_001291435.1
Coordinates 1811713..1811931 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag J6247_RS08795 Protein ID WP_000344800.1
Coordinates 1811313..1811687 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J6247_RS08785 (1806402) 1806402..1807595 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
J6247_RS08790 (1807618) 1807618..1810767 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
J6247_RS08795 (1811313) 1811313..1811687 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
J6247_RS08800 (1811713) 1811713..1811931 + 219 WP_001291435.1 HHA domain-containing protein Toxin
J6247_RS08805 (1812103) 1812103..1812654 + 552 WP_000102564.1 maltose O-acetyltransferase -
J6247_RS08810 (1812770) 1812770..1813240 + 471 WP_000136192.1 YlaC family protein -
J6247_RS08815 (1813404) 1813404..1814954 + 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
J6247_RS08820 (1814996) 1814996..1815349 - 354 WP_000878141.1 DUF1428 family protein -
J6247_RS08830 (1815728) 1815728..1816039 + 312 WP_000409911.1 MGMT family protein -
J6247_RS08835 (1816070) 1816070..1816642 - 573 WP_000779839.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T197637 WP_001291435.1 NZ_CP072322:1811713-1811931 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T197637 NZ_CP096522:c2131491-2131282 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATTGATGGTCAAGTAACGGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATTTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT197637 WP_000344800.1 NZ_CP072322:1811313-1811687 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT197637 NZ_CP096522:c2131796-2131503 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGAGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References