Detailed information of TA system TA197636

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 765809..766447
• Replicon: chromosome
• Accession: NZ_CP072322
• Organism: Escherichia coli strain EC-JS426

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: J6247_RS03735
• Protein ID: WP_000813794.1
• Coordinates: 766271..766447 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: J6247_RS03730
• Protein ID: WP_001270286.1
• Coordinates: 765809..766225 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J6247_RS03710 (760961)	760961..761902	-	942	WP_001251315.1	ABC transporter permease	-
J6247_RS03715 (761903)	761903..762916	-	1014	WP_000220416.1	ABC transporter ATP-binding protein	-
J6247_RS03720 (762934)	762934..764079	-	1146	WP_000047455.1	ABC transporter substrate-binding protein	-
J6247_RS03725 (764324)	764324..765730	-	1407	WP_000760589.1	PLP-dependent aminotransferase family protein	-
J6247_RS03730 (765809)	765809..766225	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
J6247_RS03735 (766271)	766271..766447	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
J6247_RS03740 (766669)	766669..766899	+	231	WP_000494244.1	YncJ family protein	-
J6247_RS03745 (766991)	766991..768952	-	1962	WP_001307191.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
J6247_RS03750 (769025)	769025..769561	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
J6247_RS03755 (769653)	769653..770828	+	1176	WP_001236258.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	770868..772133	1265

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T197636 WP_000813794.1 NZ_CP072322:c766447-766271 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T197636 NZ_CP096522:c2111907-2111545 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAACAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAGCAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTAAATGCAGTAGCTCATCAGAAAAATTAG

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT197636 WP_001270286.1 NZ_CP072322:c766225-765809 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT197636 NZ_CP096522:c2112074-2111904 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Toxin

• PDB: 6HPB