Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 129665..130496 Replicon chromosome
Accession NZ_CP072322
Organism Escherichia coli strain EC-JS426

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag J6247_RS00630 Protein ID WP_000854814.1
Coordinates 129665..130039 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID B3Y195
Locus tag J6247_RS00635 Protein ID WP_001285585.1
Coordinates 130128..130496 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J6247_RS00590 (125061) 125061..126227 + 1167 WP_000830150.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -
J6247_RS00595 (126346) 126346..126819 + 474 WP_001105389.1 DNA gyrase inhibitor SbmC -
J6247_RS00600 (127017) 127017..128075 + 1059 WP_001200891.1 FUSC family protein -
J6247_RS00605 (128247) 128247..128576 + 330 WP_000450409.1 DUF496 family protein -
J6247_RS00610 (128677) 128677..128811 - 135 WP_001297944.1 EutP/PduV family microcompartment system protein -
J6247_RS00615 (128931) 128931..129059 + 129 Protein_124 transposase domain-containing protein -
J6247_RS00620 (129348) 129348..129428 - 81 Protein_125 hypothetical protein -
J6247_RS00625 (129474) 129474..129668 - 195 WP_000988600.1 DUF5983 family protein -
J6247_RS00630 (129665) 129665..130039 - 375 WP_000854814.1 type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA Toxin
J6247_RS00635 (130128) 130128..130496 - 369 WP_001285585.1 type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA Antitoxin
J6247_RS00640 (130570) 130570..130791 - 222 WP_000692323.1 DUF987 domain-containing protein -
J6247_RS00645 (130854) 130854..131330 - 477 WP_001186746.1 RadC family protein -
J6247_RS00650 (131346) 131346..131825 - 480 WP_000860087.1 antirestriction protein -
J6247_RS00655 (131907) 131907..132725 - 819 WP_001234629.1 DUF932 domain-containing protein -
J6247_RS00660 (132825) 132825..133058 - 234 WP_001119729.1 DUF905 family protein -
J6247_RS00665 (133137) 133137..133592 - 456 WP_000581504.1 IrmA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T197627 WP_000854814.1 NZ_CP072322:c130039-129665 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T197627 NZ_CP096274:2733248-2733610 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAGTTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13593.48 Da        Isoelectric Point: 6.3139

>AT197627 WP_001285585.1 NZ_CP072322:c130496-130128 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT197627 NZ_CP096274:2733081-2733251 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTCATTCAA
CGAATGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LW60

References