Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 145972..147595 Replicon plasmid pE105-4
Accession NZ_CP072315
Organism Escherichia coli strain E105

Toxin (Protein)


Gene name hipA Uniprot ID H9TH67
Locus tag J0O73_RS25830 Protein ID WP_000088046.1
Coordinates 146270..147595 (+) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A5Z6V2D1
Locus tag J0O73_RS25825 Protein ID WP_024136327.1
Coordinates 145972..146265 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J0O73_RS25800 (J0O73_25800) 141844..143085 + 1242 WP_001053338.1 VWA domain-containing protein -
J0O73_RS27575 143176..143307 + 132 WP_000374059.1 hypothetical protein -
J0O73_RS25805 (J0O73_25805) 143648..143929 - 282 WP_000077926.1 hypothetical protein -
J0O73_RS25810 (J0O73_25810) 143979..144170 - 192 WP_000398480.1 hypothetical protein -
J0O73_RS25815 (J0O73_25815) 144262..144603 - 342 WP_001151575.1 hypothetical protein -
J0O73_RS25820 (J0O73_25820) 144976..145368 + 393 WP_000341066.1 hypothetical protein -
J0O73_RS25825 (J0O73_25825) 145972..146265 + 294 WP_024136327.1 helix-turn-helix transcriptional regulator Antitoxin
J0O73_RS25830 (J0O73_25830) 146270..147595 + 1326 WP_000088046.1 type II toxin-antitoxin system HipA family toxin Toxin
J0O73_RS25835 (J0O73_25835) 147656..147862 + 207 WP_000134171.1 hypothetical protein -
J0O73_RS25840 (J0O73_25840) 147964..148374 + 411 WP_000985911.1 hypothetical protein -
J0O73_RS25845 (J0O73_25845) 148387..149202 + 816 WP_053276223.1 HNH endonuclease signature motif containing protein -
J0O73_RS25850 (J0O73_25850) 149486..150183 + 698 WP_095033700.1 IS1-like element IS1B family transposase -
J0O73_RS25855 (J0O73_25855) 150227..150892 - 666 WP_000490638.1 hypothetical protein -
J0O73_RS25860 (J0O73_25860) 150950..151330 - 381 WP_001426317.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sul1 / qacE / aadA16 / dfrA27 / ARR-3 / aac(6')-Ib-cr / floR / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / mph(A) / aac(3)-IId / blaCTX-M-55 - 1..234576 234576
- flank IS/Tn - - 149486..149761 275


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(9-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50249.20 Da        Isoelectric Point: 6.1927

>T197622 WP_000088046.1 NZ_CP072315:146270-147595 [Escherichia coli]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

>T197622 NZ_CP096274:2339692-2339958 [Staphylococcus aureus]
ATGAGCAATTACACGGTTAAGATTAAAAATTCAGCGAAATCAGATTTAAAGAAAATAAAACATCCTTATTTAAAGAAGAC
ATTTATAGAAATTGTTGAGACTTTAAAAAATGATCCTTATAAAATAACACAATCCTTTGAAAAACTAGAACCTAAATATT
TAGAGCGATATTCAAGAAGAATTAACCTTCAGCACAGGGTCGTCTATACCATAGATGATCGAAATAAAGTAGTATTAATA
CTATCGGCATGGTCACATTATGATTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10946.35 Da        Isoelectric Point: 10.0925

>AT197622 WP_024136327.1 NZ_CP072315:145972-146265 [Escherichia coli]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Download         Length: 294 bp

>AT197622 NZ_CP096274:2339435-2339692 [Staphylococcus aureus]
ATGATTATCACTAGCCCTACAGAAGCGAGAAAAGATTTTTATCAATTACTAAAAAATGTTAATAATAATCACGAACCAAT
TTATATTAGTGGTAATAATGCCGAAAATAATGCTGTGATTATAGGTTTAGAAGATTGGAAAAGTATACAAGAGACAATGT
ATCTTGTATCTACTGGTACAATGGACAAAGTAAGAGAAAGAGAAAAAGATAGTAGTGGTACAACAAATATAGATGATATT
GATTGGGATAATCTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3T0VBU1

Antitoxin

Source ID Structure
AlphaFold DB A0A5Z6V2D1

References