Detailed information of TA system TA197621

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacT-ataR/DUF1778(antitoxin)
• Location: 42137..42912
• Replicon: plasmid pE105-3
• Accession: NZ_CP072314
• Organism: Escherichia coli strain E105

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A828GRZ2
• Locus tag: J0O73_RS24960
• Protein ID: WP_001569490.1
• Coordinates: 42137..42619 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: -
• Locus tag: J0O73_RS24965
• Protein ID: WP_170978511.1
• Coordinates: 42610..42912 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J0O73_RS24935 (37140)	37140..38204	+	1065	WP_100032282.1	hypothetical protein	-
J0O73_RS24940 (38641)	38641..39399	+	759	WP_100032281.1	substrate-binding domain-containing protein	-
J0O73_RS24945 (40438)	40438..41295	-	858	WP_000130992.1	incFII family plasmid replication initiator RepA	-
J0O73_RS24950 (41288)	41288..41362	-	75	WP_032241916.1	RepA leader peptide Tap	-
J0O73_RS24955 (41595)	41595..41852	-	258	WP_100032261.1	replication regulatory protein RepA	-
J0O73_RS24960 (42137)	42137..42619	-	483	WP_001569490.1	GNAT family N-acetyltransferase	Toxin
J0O73_RS24965 (42610)	42610..42912	-	303	WP_170978511.1	DUF1778 domain-containing protein	Antitoxin
J0O73_RS27400 (43236)	43236..43310	-	75	WP_233708375.1	Hok/Gef family protein	-
- (43399)	43399..43458	+	60	NuclAT_0	-	-
- (43399)	43399..43458	+	60	NuclAT_0	-	-
- (43399)	43399..43458	+	60	NuclAT_0	-	-
- (43399)	43399..43458	+	60	NuclAT_0	-	-
J0O73_RS24975 (43699)	43699..43788	-	90	Protein_39	endonuclease	-
J0O73_RS24980 (44034)	44034..44246	-	213	WP_032158816.1	hypothetical protein	-
J0O73_RS24985 (44384)	44384..44950	-	567	WP_100032258.1	fertility inhibition protein FinO	-
J0O73_RS24990 (45005)	45005..45163	-	159	Protein_42	TraX family protein	-
J0O73_RS24995 (45238)	45238..46416	+	1179	Protein_43	IS3-like element ISEc31 family transposase	-
J0O73_RS25000 (46420)	46420..47133	-	714	WP_153645691.1	type-F conjugative transfer system secretin TraK	-
J0O73_RS25005 (47120)	47120..47650	-	531	WP_001324649.1	type IV conjugative transfer system protein TraE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	espT / espC / paa	1..60072	60072

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (28-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-99)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17742.48 Da
• Isoelectric Point: 6.5977

>T197621 WP_001569490.1 NZ_CP072314:c42619-42137 [Escherichia coli]
MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQILNASRTFVICLEDTLRVVGYYSLATGSVTHAELGRSLRHN
MPNPVPVVLLGRLAVDVCTQGHGFGKWLLSDAIHRVANLAEQVGIKAVMVHAIDDDARVFYEHFGFVQSVVAPETLFYKI

Nucleotide

• Length: 483 bp

>T197621 NZ_CP096274:1212275-1212463 [Staphylococcus aureus]
ATGTCTTTACATTTTGCAATTCTGTTTTGGCTAGCATTAATTTTCTTAGTTGCCGCTACGTTTATACTCGTATTAATGAA
AAAAACTGGCAAAGAATCTAAAAAAGAGTCCTATTTAAGTTTCACTGTCATTCTCTATATTTTTGGATTCGCTATATTAA
TATACACATTTATATTTGGTGTGCTATAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11629.22 Da
• Isoelectric Point: 4.4569

>AT197621 WP_170978511.1 NZ_CP072314:c42912-42610 [Escherichia coli]
MEVVMPTTANIRKTPRENQINIRATDEERAVIDYAASLVNKNRTDFIIELAYQEAKNIILDQRLFVLDDECYDSFITQLE
APVQNAEGRERLMAVKPEWK

Nucleotide

• Length: 303 bp

>AT197621 NZ_CP096274:1211986-1212246 [Staphylococcus aureus]
ATGAATAACATTTTGTTAAATGCTATCAATATAGTTATTACTACCACTTTTGTTATCTTTAATATTTTAATCACATATAA
TAAAGATTTAGATGATTTATGTTGGCTCCTGCCTGGTATTATCATTTGTGGTGTGATACTCATCGTATCCTTTACCATTG
CAATGATAACTAAAAACTGGTTAAGTGAAATATTATTTTTTATAAATATCGTACTCGTTCTCTATTACATTTATCCTATT
TTTTATAGTTTTATAGGTTAA

Structures

Toxin

• AlphaFold DB: A0A828GRZ2