Detailed information of TA system TA197617

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1212104..1213021
• Replicon: chromosome
• Accession: NZ_CP072311
• Organism: Bacillus velezensis strain SC60

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: J6B42_RS06440
• Protein ID: WP_025851786.1
• Coordinates: 1212275..1213021 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: J6B42_RS06435
• Protein ID: WP_003154807.1
• Coordinates: 1212104..1212274 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J6B42_RS06395 (J6B42_06345)	1207335..1208957	+	1623	WP_003154821.1	pyocin knob domain-containing protein	-
J6B42_RS06400 (J6B42_06350)	1208970..1209341	+	372	WP_003154820.1	XkdW family protein	-
J6B42_RS06405 (J6B42_06355)	1209347..1209544	+	198	WP_003154819.1	XkdX family protein	-
J6B42_RS06410 (J6B42_06360)	1209601..1210362	+	762	WP_025851792.1	hypothetical protein	-
J6B42_RS06415 (J6B42_06365)	1210414..1210677	+	264	WP_003154815.1	hemolysin XhlA family protein	-
J6B42_RS06420 (J6B42_06370)	1210691..1210954	+	264	WP_025851790.1	phage holin	-
J6B42_RS06425 (J6B42_06375)	1210968..1211846	+	879	WP_025851788.1	N-acetylmuramoyl-L-alanine amidase	-
J6B42_RS06430	1211882..1212007	-	126	WP_003154809.1	hypothetical protein	-
J6B42_RS06435 (J6B42_06380)	1212104..1212274	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
J6B42_RS06440 (J6B42_06385)	1212275..1213021	-	747	WP_025851786.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
J6B42_RS06445 (J6B42_06390)	1213126..1214124	-	999	WP_003154805.1	inorganic phosphate transporter	-
J6B42_RS06450 (J6B42_06395)	1214137..1214754	-	618	WP_003154804.1	DUF47 domain-containing protein	-
J6B42_RS06455 (J6B42_06400)	1215040..1216356	-	1317	WP_003154801.1	amino acid permease	-
J6B42_RS06460 (J6B42_06405)	1216679..1217629	+	951	WP_015388352.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1177840..1270384	92544

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29048.53 Da
• Isoelectric Point: 4.7003

>T197617 WP_025851786.1 NZ_CP072311:c1213021-1212275 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVVGAMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYGEKMNVTASL
CEYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQDRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T197617 NZ_CP096272:2733248-2733610 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAGTTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT197617 WP_003154807.1 NZ_CP072311:c1212274-1212104 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT197617 NZ_CP096272:2733081-2733251 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTCATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: I2HQ14