Detailed information of TA system TA197616

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 475002..475639
• Replicon: chromosome
• Accession: NZ_CP072311
• Organism: Bacillus velezensis strain SC60

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: J6B42_RS02450
• Protein ID: WP_003156187.1
• Coordinates: 475289..475639 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: J6B42_RS02445
• Protein ID: WP_003156188.1
• Coordinates: 475002..475283 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J6B42_RS02425 (J6B42_02395)	471368..471967	-	600	WP_003156193.1	rhomboid family intramembrane serine protease	-
J6B42_RS02430 (J6B42_02400)	472060..472425	+	366	WP_014304402.1	holo-ACP synthase	-
J6B42_RS02435 (J6B42_02405)	472589..473596	+	1008	WP_003156191.1	outer membrane lipoprotein carrier protein LolA	-
J6B42_RS02440 (J6B42_02410)	473713..474882	+	1170	WP_025853726.1	alanine racemase	-
J6B42_RS02445 (J6B42_02415)	475002..475283	+	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
J6B42_RS02450 (J6B42_02420)	475289..475639	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
J6B42_RS02455 (J6B42_02425)	475757..476578	+	822	WP_003156182.1	STAS domain-containing protein	-
J6B42_RS02460 (J6B42_02430)	476583..476948	+	366	WP_003156180.1	RsbT antagonist protein RsbS	-
J6B42_RS02465 (J6B42_02435)	476951..477352	+	402	WP_003156178.1	anti-sigma regulatory factor	-
J6B42_RS02470 (J6B42_02440)	477364..478371	+	1008	WP_025853725.1	PP2C family protein-serine/threonine phosphatase	-
J6B42_RS02475 (J6B42_02445)	478435..478764	+	330	WP_003156176.1	anti-sigma factor antagonist RsbV	-
J6B42_RS02480 (J6B42_02450)	478761..479243	+	483	WP_003156173.1	anti-sigma B factor RsbW	-
J6B42_RS02485 (J6B42_02455)	479209..479997	+	789	WP_003156171.1	RNA polymerase sigma factor SigB	-
J6B42_RS02490 (J6B42_02460)	479997..480599	+	603	WP_003156170.1	PP2C family serine/threonine-protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T197616 WP_003156187.1 NZ_CP072311:475289-475639 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T197616 NZ_CP096272:2713661-2713870 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGATGGTCAAGTAACTGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTAATTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT197616 WP_003156188.1 NZ_CP072311:475002-475283 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT197616 NZ_CP096272:2713356-2713649 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCGATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5