Detailed information of TA system TA197615

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 3377350..3378267
• Replicon: chromosome
• Accession: NZ_CP072310
• Organism: Bacillus velezensis strain AD8

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: J4774_RS17000
• Protein ID: WP_025851786.1
• Coordinates: 3377521..3378267 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: J4774_RS16995
• Protein ID: WP_003154807.1
• Coordinates: 3377350..3377520 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J4774_RS16960	3372572..3374203	+	1632	WP_139890282.1	pyocin knob domain-containing protein	-
J4774_RS16965	3374216..3374587	+	372	WP_003154820.1	XkdW family protein	-
J4774_RS16970	3374593..3374790	+	198	WP_003154819.1	XkdX family protein	-
J4774_RS16975	3374847..3375608	+	762	WP_139890281.1	phage portal protein	-
J4774_RS16980	3375660..3375923	+	264	WP_003154815.1	hemolysin XhlA family protein	-
J4774_RS16985	3375937..3376200	+	264	WP_003154813.1	phage holin	-
J4774_RS16990	3376214..3377092	+	879	WP_014304858.1	N-acetylmuramoyl-L-alanine amidase	-
J4774_RS19355	3377128..3377253	-	126	WP_003154809.1	hypothetical protein	-
J4774_RS16995	3377350..3377520	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
J4774_RS17000	3377521..3378267	-	747	WP_025851786.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
J4774_RS17005	3378371..3379369	-	999	WP_003154805.1	inorganic phosphate transporter	-
J4774_RS17010	3379382..3379999	-	618	WP_003154804.1	DUF47 domain-containing protein	-
J4774_RS17015	3380285..3381601	-	1317	WP_003154801.1	amino acid permease	-
J4774_RS17020	3381924..3382874	+	951	WP_015388352.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29048.53 Da
• Isoelectric Point: 4.7003

>T197615 WP_025851786.1 NZ_CP072310:c3378267-3377521 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVVGAMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYGEKMNVTASL
CEYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQDRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T197615 NZ_CP096272:2656321-2656425 [Staphylococcus aureus]
TTGTTACTCCTAGAAAGGACTAGCATGTCTGATTTTGAAATGCTTATGGTTGTATTAACAATCATTGGTTTAGTATTGAT
TAGTACTCAAGACCATAAAAAATAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT197615 WP_003154807.1 NZ_CP072310:c3377520-3377350 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT197615 NZ_CP096272:c2656584-2656420 [Staphylococcus aureus]
GTAGTAAGTAGAAGCAAAAGATGAAAATCATTAACTCTTGAAACACAAAAAGGGCAACACTCGGAAACATGTTACCCTAA
TGAGCCCGTTAAAAAGACGGTGACCTTATATTTTATTTAAAAATAGCCTTCAAAAATGCCGGTCAAAGCGAATAGAAGGT
TATTT

Structures

Antitoxin

• AlphaFold DB: I2HQ14