Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2623563..2624200 Replicon chromosome
Accession NZ_CP072310
Organism Bacillus velezensis strain AD8

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag J4774_RS13025 Protein ID WP_003156187.1
Coordinates 2623850..2624200 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag J4774_RS13020 Protein ID WP_003156188.1
Coordinates 2623563..2623844 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J4774_RS13000 2619928..2620527 - 600 WP_003156193.1 rhomboid family intramembrane serine protease -
J4774_RS13005 2620620..2620985 + 366 WP_014304402.1 holo-ACP synthase -
J4774_RS13010 2621150..2622157 + 1008 WP_003156191.1 outer membrane lipoprotein carrier protein LolA -
J4774_RS13015 2622274..2623443 + 1170 WP_003156189.1 alanine racemase -
J4774_RS13020 2623563..2623844 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
J4774_RS13025 2623850..2624200 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
J4774_RS13030 2624318..2625139 + 822 WP_003156182.1 STAS domain-containing protein -
J4774_RS13035 2625144..2625509 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
J4774_RS13040 2625512..2625913 + 402 WP_003156178.1 anti-sigma regulatory factor -
J4774_RS13045 2625925..2626932 + 1008 WP_003156177.1 PP2C family protein-serine/threonine phosphatase -
J4774_RS13050 2626996..2627325 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
J4774_RS13055 2627322..2627804 + 483 WP_003156173.1 anti-sigma B factor RsbW -
J4774_RS13060 2627770..2628558 + 789 WP_003156171.1 RNA polymerase sigma factor SigB -
J4774_RS13065 2628558..2629160 + 603 WP_003156170.1 PP2C family serine/threonine-protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T197614 WP_003156187.1 NZ_CP072310:2623850-2624200 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T197614 NZ_CP096272:2396558-2396824 [Staphylococcus aureus]
ATGGCTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACGATAAAAAAATGGT
GAAGAAGATTAATGAGTTACTTAAAAGTATTGACAGAAATGGTGCATTGGAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGGTATTATAGTAGACGTATCAATCACGAACATAGATTGGTTTATACAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCATTATTAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT197614 WP_003156188.1 NZ_CP072310:2623563-2623844 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT197614 NZ_CP096272:2396307-2396558 [Staphylococcus aureus]
ATGATTATTAAAAATTATTCATACGCTCGACAGAATTTAAAGGCACTTATGACAAAAGTAAATGATGATAGTGATATGGT
AACTGTAACATCTACTGATGATAAAAACGTAGTAATCATGTCAGAATCAGATTATAACTCCATGATGGAAACACTTTACC
TCCAACAGAACCCAAATAATGCTGAACACTTAGCTCAATCAATTGCAGATCTAGAACGTGGGAAAACTATAACGAAAGAT
ATAGATGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References