Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 2699..3288 Replicon plasmid pXAG27.2526
Accession NZ_CP072264
Organism Xanthomonas citri pv. glycines CFBP 2526

Toxin (Protein)


Gene name pemK Uniprot ID Q32X98
Locus tag XcgCFBP2526_RS22745 Protein ID WP_012477455.1
Coordinates 2699..3031 (-) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID Q32X99
Locus tag XcgCFBP2526_RS22750 Protein ID WP_012477454.1
Coordinates 3031..3288 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
XcgCFBP2526_RS22740 (XcgCFBP2526_22740) 1792..2610 + 819 WP_012477456.1 hypothetical protein -
XcgCFBP2526_RS22745 (XcgCFBP2526_22745) 2699..3031 - 333 WP_012477455.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
XcgCFBP2526_RS22750 (XcgCFBP2526_22750) 3031..3288 - 258 WP_012477454.1 plasmid stable inheritance protein I Antitoxin
XcgCFBP2526_RS22755 (XcgCFBP2526_22755) 3554..3781 + 228 WP_012477437.1 hypothetical protein -
XcgCFBP2526_RS22760 (XcgCFBP2526_22760) 3778..4422 + 645 WP_012477436.1 recombinase family protein -
XcgCFBP2526_RS22765 (XcgCFBP2526_22765) 4489..5139 + 651 WP_012477435.1 ParA family protein -
XcgCFBP2526_RS22770 (XcgCFBP2526_22770) 5139..5393 + 255 WP_012477434.1 hypothetical protein -
XcgCFBP2526_RS22775 (XcgCFBP2526_22775) 5616..5774 + 159 WP_154666355.1 hypothetical protein -
XcgCFBP2526_RS22780 (XcgCFBP2526_22780) 5771..6577 + 807 WP_016851361.1 serine protease -
XcgCFBP2526_RS22785 (XcgCFBP2526_22785) 6643..7563 + 921 WP_012477431.1 replication protein RepA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..26618 26618


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-108)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11581.24 Da        Isoelectric Point: 9.1684

>T197555 WP_012477455.1 NZ_CP072264:c3031-2699 [Xanthomonas citri pv. glycines CFBP 2526]
MERGDIYLVSLDPTSGHEQQGTRPVLIVSPGPFNRLTKTPVVLPITTGGNFARTAGFTVSLTGGGTKTTGVVRCDQPRAL
DIGSRNGRKLESVSAAITDEVMAKLATILE

Download         Length: 333 bp

>T197555 NZ_CP096242:c4913071-4912787 [Klebsiella pneumoniae subsp. pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 8951.34 Da        Isoelectric Point: 5.5491

>AT197555 WP_012477454.1 NZ_CP072264:c3288-3031 [Xanthomonas citri pv. glycines CFBP 2526]
MHTTNLRKVGGSVMLAVPPALLDVLHIGAGAKVGLAVDNGRLVVEPHARPRYTLADLLAQCDASAELASEDREWLDAKPV
GGELL

Download         Length: 258 bp

>AT197555 NZ_CP096242:c4913303-4913061 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7Z6Z9Y5

Antitoxin

Source ID Structure
AlphaFold DB A0A1D9EPT3

References