Detailed information of TA system TA197548

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 36717..38268
• Replicon: plasmid pMMCAT_PdisCL06T03
• Accession: NZ_CP072233
• Organism: Parabacteroides distasonis strain CL06T03C10

Toxin (Protein)

• Gene name: HipA3
• Uniprot ID: -
• Locus tag: INE86_RS22005
• Protein ID: WP_249931960.1
• Coordinates: 36717..37961 (-)
• Length: 415 a.a.

Antitoxin (Protein)

• Gene name: HipB3
• Uniprot ID: -
• Locus tag: INE86_RS22010
• Protein ID: WP_008782417.1
• Coordinates: 37954..38268 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
INE86_RS21980 (INE86_04481)	31872..33398	-	1527	WP_005868817.1	type II toxin-antitoxin system HipA family toxin	-
INE86_RS21985 (INE86_04482)	33388..33708	-	321	WP_005868815.1	helix-turn-helix transcriptional regulator	-
INE86_RS21990 (INE86_04483)	33808..34224	-	417	WP_249931959.1	hypothetical protein	-
INE86_RS21995 (INE86_04484)	34263..35018	-	756	WP_004288784.1	IS21-like element helper ATPase IstB	-
INE86_RS22000 (INE86_04485)	35030..36592	-	1563	WP_249931902.1	IS21 family transposase	-
INE86_RS22005 (INE86_04486)	36717..37961	-	1245	WP_249931960.1	type II toxin-antitoxin system HipA family toxin	Toxin
INE86_RS22010 (INE86_04487)	37954..38268	-	315	WP_008782417.1	helix-turn-helix domain-containing protein	Antitoxin
INE86_RS22860	38434..38593	+	160	Protein_38	helix-turn-helix transcriptional regulator	-
INE86_RS22015 (INE86_04488)	38720..40000	+	1281	WP_005868811.1	ATP-binding protein	-
INE86_RS22020 (INE86_04489)	40306..41367	-	1062	WP_005868810.1	LuxR C-terminal-related transcriptional regulator	-
INE86_RS22025 (INE86_04490)	41673..42905	+	1233	WP_005868809.1	DUF3575 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..104793	104793

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (7-120)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-65)

Sequences

Toxin

Protein

• Length: 415 a.a.
• Molecular weight: 47969.36 Da
• Isoelectric Point: 6.3231

>T197548 WP_249931960.1 NZ_CP072233:c37961-36717 [Parabacteroides distasonis]
MNDLIVDVKLWGESVGSLYWEKESNAALFDYERKFIRSGLDISPIIMPISQYRNTPYRFLENRTDCFKGLPGLFADSLPD
TFGNQIINEWFASKGLSGEEITPLDRLCYVGKRGMGALEFEPSSPINGMNESSVLHIEELTELAKSVFTDRMAFQVQLRQ
EGRNILDILKVGTSAGGAKPKAIIAYNDITGEVRSGQVKAPEGFGYWLLKFDGGKYSEHTQITDNPQGIGNIEYAYHRMA
KACGIDMMECRLLQEKESYHFMTRRFDRMEDGEKIHVQTLAGLAHYDRDQRHSYEEIFRIMRQMNLPYPEQEELYRRMVF
NVMSRNHDDHSKNLSFLMDRQGKWKLAPAYDLCYSYTPGGKWTNRHQLSLNGKQDNFTMEDLQKVGENMGIRKHKQIIEE
IQETVSHWHETCRY

Nucleotide

• Length: 1245 bp

>T197548 NZ_CP096242:327908-328276 [Klebsiella pneumoniae subsp. pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACTGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11951.02 Da
• Isoelectric Point: 10.7982

>AT197548 WP_008782417.1 NZ_CP072233:c38268-37954 [Parabacteroides distasonis]
MWNEMSNPAVLMRIGQRIKETRIRQHITQEELATASGVSPLTVANIEKGKSVSLLLFISVLRSLGLLENLEQLVPEIRVS
PIELKKLQGKKRYRVRHLKQDNHE

Nucleotide

• Length: 315 bp

>AT197548 NZ_CP096242:327690-327911 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA