Detailed information of TA system TA197546

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Fic(toxin)
• Location: 2927502..2928070
• Replicon: chromosome
• Accession: NZ_CP072209
• Organism: Streptomyces fradiae strain ZXY017

Toxin (Protein)

• Gene name: doc
• Uniprot ID: A0A1D8G0Y6
• Locus tag: J5J01_RS12760
• Protein ID: WP_069976589.1
• Coordinates: 2927699..2928070 (+)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: J5J01_RS12755
• Protein ID: WP_031131122.1
• Coordinates: 2927502..2927699 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J5J01_RS12720 (J5J01_12640)	2922992..2923618	+	627	WP_249467389.1	hypothetical protein	-
J5J01_RS12725 (J5J01_12645)	2923684..2924043	-	360	WP_037937727.1	hypothetical protein	-
J5J01_RS12730 (J5J01_12650)	2924040..2924366	-	327	WP_031131133.1	hypothetical protein	-
J5J01_RS12735 (J5J01_12655)	2924538..2924798	+	261	WP_249467392.1	winged helix-turn-helix domain-containing protein	-
J5J01_RS12740 (J5J01_12660)	2924930..2925157	+	228	WP_249467394.1	winged helix-turn-helix domain-containing protein	-
J5J01_RS12745 (J5J01_12665)	2925248..2925748	+	501	WP_031131127.1	VOC family protein	-
J5J01_RS12750 (J5J01_12670)	2925995..2927470	+	1476	WP_249467397.1	MFS transporter	-
J5J01_RS12755 (J5J01_12675)	2927502..2927699	+	198	WP_031131122.1	Arc family DNA-binding protein	Antitoxin
J5J01_RS12760 (J5J01_12680)	2927699..2928070	+	372	WP_069976589.1	Fic family protein	Toxin
J5J01_RS12765 (J5J01_12685)	2928245..2930029	+	1785	WP_078915563.1	DEAD/DEAH box helicase	-
J5J01_RS12770 (J5J01_12690)	2930666..2931310	+	645	WP_031131116.1	helix-turn-helix domain-containing protein	-
J5J01_RS12775 (J5J01_12695)	2931432..2932229	+	798	WP_031131114.1	S16 family serine protease	-
J5J01_RS12780 (J5J01_12700)	2932301..2932786	-	486	WP_031131112.1	Lrp/AsnC family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (20-80)

Antitoxin

• Arc-like DNA binding domain (5-45)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13675.61 Da
• Isoelectric Point: 5.1069

>T197546 WP_069976589.1 NZ_CP072209:2927699-2928070 [Streptomyces fradiae]
MRYLTLPELLNLAERLGTPDVRDYGLLDSALARPRSSVFGQDAYPDVWQKAAALMESIARNHGLVDGNKPLAWYATWVFL
HVNGHPLDGAFDVDEAERFVLDVCQGALDVPKIAAQLPRFAVR

Nucleotide

• Length: 372 bp

>T197546 NZ_CP096239:c158104-157661 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 7412.28 Da
• Isoelectric Point: 5.1114

>AT197546 WP_031131122.1 NZ_CP072209:2927502-2927699 [Streptomyces fradiae]
MAGLNVRFSEEELTALRERAEAEGRSMQAFAHDAVMRSVNEHSRLFDEAAEHVLKVSEELNRRLA

Nucleotide

• Length: 198 bp

>AT197546 NZ_CP096239:c158331-158101 [Klebsiella pneumoniae subsp. pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Toxin

• AlphaFold DB: A0A1D8G0Y6