Detailed information of TA system TA197545

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-Phd
• Location: 914253..914778
• Replicon: chromosome
• Accession: NZ_CP072209
• Organism: Streptomyces fradiae strain ZXY017

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: J5J01_RS03765
• Protein ID: WP_069977857.1
• Coordinates: 914515..914778 (+)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A1D8G5V8
• Locus tag: J5J01_RS03760
• Protein ID: WP_069977861.1
• Coordinates: 914253..914522 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J5J01_RS03735 (J5J01_03695)	910391..910642	+	252	WP_031128870.1	DUF397 domain-containing protein	-
J5J01_RS03740 (J5J01_03700)	910809..911705	+	897	WP_249465755.1	helix-turn-helix transcriptional regulator	-
J5J01_RS03745 (J5J01_03705)	911837..912643	-	807	WP_249465758.1	SAM-dependent methyltransferase	-
J5J01_RS03750 (J5J01_03710)	912807..913304	-	498	WP_069977863.1	hypothetical protein	-
J5J01_RS03755	913753..914030	+	278	Protein_736	hypothetical protein	-
J5J01_RS03760 (J5J01_03715)	914253..914522	+	270	WP_069977861.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
J5J01_RS03765 (J5J01_03720)	914515..914778	+	264	WP_069977857.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
J5J01_RS03770 (J5J01_03725)	915477..917096	-	1620	WP_249465764.1	endonuclease NucS	-
J5J01_RS03775 (J5J01_03730)	917693..918154	-	462	WP_079140233.1	hypothetical protein	-
J5J01_RS03780 (J5J01_03735)	918151..918687	-	537	WP_249465767.1	NUDIX domain-containing protein	-
J5J01_RS03785 (J5J01_03740)	918689..918868	-	180	Protein_742	tetratricopeptide repeat protein	-
J5J01_RS03790 (J5J01_03745)	918903..919595	+	693	WP_249465770.1	site-specific integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (8-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (9-58)

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 10011.45 Da
• Isoelectric Point: 9.5565

>T197545 WP_069977857.1 NZ_CP072209:914515-914778 [Streptomyces fradiae]
VSDYRTVFRPEAQAELRKVPRDMALRILAKLAELESNPLGFNTTALVSQPERRRLRVGDYRVVYTIDNGELVVWVVHVGH
RSTVYDT

Nucleotide

• Length: 264 bp

>T197545 NZ_CP096239:c105464-104994 [Klebsiella pneumoniae subsp. pneumoniae]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
TTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACGCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATCCAAAGCGCAAACATGGAT
GAATTACCGGGCAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAACTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10280.78 Da
• Isoelectric Point: 5.2172

>AT197545 WP_069977861.1 NZ_CP072209:914253-914522 [Streptomyces fradiae]
MTQPLPIESIRDVRAHLAEVVERADRDDVPTVITRRGKQVAAVVSIEVLRKYQEWEEREINRIIDERMANPAPGIPIEHI
MRETLARGE

Nucleotide

• Length: 270 bp

>AT197545 NZ_CP096239:c105904-105461 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAAAACATTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
CGTAGCGCTTCTGGGCCAAATCAATGAAGGCCTGGACGGGAAGGTGGCGAATCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCGACCACGTTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGGATTATCCGCGTCATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAAGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAGACAGGAGCGTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Structures

Antitoxin

• AlphaFold DB: A0A1D8G5V8