Detailed information of TA system TA197544

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RelB
• Location: 21695..22261
• Replicon: chromosome
• Accession: NZ_CP072209
• Organism: Streptomyces fradiae strain ZXY017

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A1Y2NPN0
• Locus tag: J5J01_RS00115
• Protein ID: WP_031129655.1
• Coordinates: 21695..21976 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: J5J01_RS00120
• Protein ID: WP_223844470.1
• Coordinates: 21980..22261 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J5J01_RS00085 (J5J01_00075)	17070..17489	+	420	WP_031129647.1	roadblock/LC7 domain-containing protein	-
J5J01_RS00090 (J5J01_00080)	17486..17842	+	357	WP_031129648.1	DUF742 domain-containing protein	-
J5J01_RS00095 (J5J01_00085)	17823..18440	+	618	WP_069978394.1	ATP/GTP-binding protein	-
J5J01_RS00100 (J5J01_00090)	18437..19702	+	1266	WP_031129652.1	cytochrome P450	-
J5J01_RS00105 (J5J01_00095)	19902..21137	-	1236	WP_249465206.1	cytochrome P450	-
J5J01_RS00110 (J5J01_00100)	21261..21617	-	357	WP_031129654.1	3'-5' exonuclease	-
J5J01_RS00115 (J5J01_00105)	21695..21976	-	282	WP_031129655.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
J5J01_RS00120 (J5J01_00110)	21980..22261	-	282	WP_223844470.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
J5J01_RS00125 (J5J01_00115)	22258..22797	-	540	WP_249465208.1	ATP-binding protein	-
J5J01_RS00130 (J5J01_00120)	23000..23866	+	867	WP_257787048.1	helix-turn-helix transcriptional regulator	-
J5J01_RS00135 (J5J01_00125)	23863..24045	+	183	WP_249465210.1	DUF397 domain-containing protein	-
J5J01_RS00140 (J5J01_00130)	24416..24895	-	480	WP_249465211.1	ASCH domain-containing protein	-
J5J01_RS00145 (J5J01_00135)	24892..26952	-	2061	WP_249465212.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-89)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (8-70)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10844.55 Da
• Isoelectric Point: 10.5709

>T197544 WP_031129655.1 NZ_CP072209:c21976-21695 [Streptomyces fradiae]
VGYVTRFTPHAQRDMLKVPRPDALRILYRLAELQKAIDGGDTTAFDIKALRSHHARWRLRVGDYRVVYTVEDGQLIVWVL
AVGNRREVYRQIP

Nucleotide

• Length: 282 bp

>T197544 NZ_CP096239:c60816-60505 [Klebsiella pneumoniae subsp. pneumoniae]
ATGCACGTAATTTCAAAAGAGCCTTTTGAGGAAGCGGCAAAGCGATATCCCAACGATTCGTTAGCCATTCGGGCGTTGTA
CCGCTTAGTGCGCGAGACGGACTTCTCTTCTCCAGCTGAGATGCTAACTCTGATACCGAGCCTGGATAATTTTAAGTACA
GGAATAAGTGGTGGGTTTTGGATGTAGGGGGCAACAACTTAAGGGTTATCGCCTATATCAATTTCGTAAATAAGCGCTTC
TACGTGAAGCACATCGCAACCCACGCTGATTACGACAAGTTGACCCGCTATTACAGGGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10566.75 Da
• Isoelectric Point: 4.3048

>AT197544 WP_223844470.1 NZ_CP072209:c22261-21980 [Streptomyces fradiae]
MSEPIVESMAEVRSHLADVIDRARREETPTIITRRGKQEAVVIDIEEYQRLRRLAEDAEEAWLNRLADEAESEGTRGSVS
LEEMAALLRTDQG

Nucleotide

• Length: 282 bp

>AT197544 NZ_CP096239:c60508-60089 [Klebsiella pneumoniae subsp. pneumoniae]
ATGATTACCGACACTGCAAAAGCCATTGAAGCAACCAAACAACTCGTAGCTGCCGTCCCCTTCCTAGGGGGCAGTTCTTC
CGAGAGCGATTACCGCGAAGCGATGGAGCTGGTGGATTATCTGATCGAGAACGACGACGAGAACCCACTTATTGACTTCC
TGGCGAGTAAGATTGCCGACTATGAAGATAACAGCCCTCGTTTCGCTGAGTTCAACAAAGCTATTGCTGAAATACCAGTC
GGCGTTGCACTTCTGCGAACCCTGATTGACCAGCACAAGCTGTCTTATTCAGACCTTAAGGATGAGATCGGATCTAAATC
ATTGGTCAGCCAAATCCTGTCAGGGCAGCGCTCTTTGACCATCACTCACATAAAAGCTCTTTCCGCGCGCTTCGGTGTAA
AACCTGAGTGGTTCCTATAA

Structures

Toxin

• AlphaFold DB: A0A1Y2NPN0