Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 3567186..3568023 Replicon chromosome
Accession NZ_CP072204
Organism Escherichia coli strain LWY6

Toxin (Protein)


Gene name itaT Uniprot ID Q3Z4X7
Locus tag J4T81_RS17250 Protein ID WP_000227784.1
Coordinates 3567481..3568023 (+) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag J4T81_RS17245 Protein ID WP_001297137.1
Coordinates 3567186..3567497 (+) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J4T81_RS17220 (3562206) 3562206..3563153 + 948 WP_001239441.1 cytochrome o ubiquinol oxidase subunit II -
J4T81_RS17225 (3563175) 3563175..3565166 + 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
J4T81_RS17230 (3565156) 3565156..3565770 + 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
J4T81_RS17235 (3565770) 3565770..3566099 + 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
J4T81_RS17240 (3566111) 3566111..3567001 + 891 WP_000971336.1 heme o synthase -
J4T81_RS17245 (3567186) 3567186..3567497 + 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
J4T81_RS17250 (3567481) 3567481..3568023 + 543 WP_000227784.1 GNAT family N-acetyltransferase Toxin
J4T81_RS17255 (3568079) 3568079..3569014 - 936 WP_001297127.1 tetratricopeptide repeat protein -
J4T81_RS17260 (3569422) 3569422..3570786 + 1365 WP_001000978.1 MFS transporter -
J4T81_RS17265 (3570914) 3570914..3571405 - 492 WP_001138904.1 nucleotide binding protein YajQ -
J4T81_RS17270 (3571573) 3571573..3572484 + 912 WP_000705847.1 2-dehydropantoate 2-reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19805.02 Da        Isoelectric Point: 8.3395

>T197539 WP_000227784.1 NZ_CP072204:3567481-3568023 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

>T197539 NZ_CP096238:2071365-2071467 [Klebsiella pneumoniae subsp. pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT197539 WP_001297137.1 NZ_CP072204:3567186-3567497 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT197539 NZ_CP096238:c2071474-2071329 [Klebsiella pneumoniae subsp. pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7BYY

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References