Detailed information of TA system TA197536

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2527227..2527865
• Replicon: chromosome
• Accession: NZ_CP072204
• Organism: Escherichia coli strain LWY6

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: J4T81_RS12190
• Protein ID: WP_000813794.1
• Coordinates: 2527689..2527865 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: J4T81_RS12185
• Protein ID: WP_001270286.1
• Coordinates: 2527227..2527643 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J4T81_RS12165 (2522379)	2522379..2523320	-	942	WP_001251319.1	ABC transporter permease	-
J4T81_RS12170 (2523321)	2523321..2524334	-	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
J4T81_RS12175 (2524352)	2524352..2525497	-	1146	WP_000047456.1	ABC transporter substrate-binding protein	-
J4T81_RS12180 (2525742)	2525742..2527148	-	1407	WP_000760629.1	PLP-dependent aminotransferase family protein	-
J4T81_RS12185 (2527227)	2527227..2527643	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
J4T81_RS12190 (2527689)	2527689..2527865	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
J4T81_RS12195 (2528087)	2528087..2528317	+	231	WP_000494244.1	YncJ family protein	-
J4T81_RS12200 (2528409)	2528409..2530370	-	1962	WP_001307191.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
J4T81_RS12205 (2530443)	2530443..2530979	-	537	WP_000429141.1	DNA-binding transcriptional regulator SutR	-
J4T81_RS12210 (2531071)	2531071..2532246	+	1176	WP_001236319.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2532286..2533551	1265

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T197536 WP_000813794.1 NZ_CP072204:c2527865-2527689 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T197536 NZ_CP096238:329009-329377 [Klebsiella pneumoniae subsp. pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACTGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT197536 WP_001270286.1 NZ_CP072204:c2527643-2527227 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT197536 NZ_CP096238:328791-329012 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Toxin

• PDB: 6HPB