Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 267641..268227 Replicon chromosome
Accession NZ_CP072204
Organism Escherichia coli strain LWY6

Toxin (Protein)


Gene name hicA Uniprot ID A0A0H0RQZ4
Locus tag J4T81_RS01180 Protein ID WP_042014162.1
Coordinates 267641..267871 (+) Length 77 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag J4T81_RS01185 Protein ID WP_000593555.1
Coordinates 267868..268227 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J4T81_RS01170 (263678) 263678..266413 + 2736 WP_000149165.1 ribosome-associated ATPase/putative transporter RbbA -
J4T81_RS01175 (266413) 266413..267537 + 1125 WP_001314210.1 ABC transporter permease -
J4T81_RS01180 (267641) 267641..267871 + 231 WP_042014162.1 type II toxin-antitoxin system HicA family toxin Toxin
J4T81_RS01185 (267868) 267868..268227 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
J4T81_RS01190 (268347) 268347..268748 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
J4T81_RS01195 (268754) 268754..269560 - 807 WP_000173666.1 nickel import ATP-binding protein NikE -
J4T81_RS01200 (269557) 269557..270321 - 765 WP_001136236.1 nickel import ATP-binding protein NikD -
J4T81_RS01205 (270321) 270321..271154 - 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
J4T81_RS01210 (271151) 271151..272095 - 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(15-65)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 77 a.a.        Molecular weight: 8419.79 Da        Isoelectric Point: 8.5518

>T197519 WP_042014162.1 NZ_CP072204:267641-267871 [Escherichia coli]
MDQLFKSPLPQGIKWTDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP

Download         Length: 231 bp

>T197519 NZ_CP096232:c6201-5896 [Klebsiella pneumoniae subsp. pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGATGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT197519 WP_000593555.1 NZ_CP072204:267868-268227 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT197519 NZ_CP096232:c6421-6203 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H0RQZ4

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References