Detailed information of TA system TA197518

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 3752050..3752534
• Replicon: chromosome
• Accession: NZ_CP072203
• Organism: Gordonia polyisoprenivorans strain R9

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: J6U32_RS16865
• Protein ID: WP_079932148.1
• Coordinates: 3752050..3752382 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: J6U32_RS16870
• Protein ID: WP_020171194.1
• Coordinates: 3752379..3752534 (-)
• Length: 52 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J6U32_RS16840 (J6U32_16840)	3747494..3747733	-	240	WP_208791340.1	hypothetical protein	-
J6U32_RS16845 (J6U32_16845)	3748298..3749608	+	1311	WP_208791341.1	ISL3 family transposase	-
J6U32_RS16850 (J6U32_16850)	3749770..3750570	+	801	WP_208791342.1	hypothetical protein	-
J6U32_RS16855 (J6U32_16855)	3750814..3751080	+	267	WP_208791343.1	hypothetical protein	-
J6U32_RS16860 (J6U32_16860)	3751245..3751736	-	492	WP_208791344.1	hypothetical protein	-
J6U32_RS16865 (J6U32_16865)	3752050..3752382	-	333	WP_079932148.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
J6U32_RS16870 (J6U32_16870)	3752379..3752534	-	156	WP_020171194.1	antitoxin MazE family protein	Antitoxin
J6U32_RS16875 (J6U32_16875)	3752745..3753086	-	342	WP_208791345.1	type II toxin-antitoxin system VapC family toxin	-
J6U32_RS16880 (J6U32_16880)	3753226..3753405	-	180	WP_014362243.1	hypothetical protein	-
J6U32_RS16885 (J6U32_16885)	3753513..3754304	-	792	WP_208791346.1	ABC transporter permease	-
J6U32_RS16890 (J6U32_16890)	3754351..3755619	-	1269	WP_208791347.1	APC family permease	-
J6U32_RS27455	3755747..3756115	-	369	Protein_3357	HAD-IA family hydrolase	-
J6U32_RS27460	3756046..3756453	-	408	WP_244332058.1	hypothetical protein	-
J6U32_RS16905 (J6U32_16905)	3756979..3757179	+	201	WP_208796356.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-108)

Antitoxin

• Antitoxin MazE-like (1-49)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 12029.88 Da
• Isoelectric Point: 6.3254

>T197518 WP_079932148.1 NZ_CP072203:c3752382-3752050 [Gordonia polyisoprenivorans]
VIRGEIWTVAGGVYASKPRPAVIVQDDLFDSTLSVVVAPITSQLIDAPLLRIRIPGDRDSISGLERDSDVMVDKLTAVRR
SNVQTRVGRLTSDQLVEVERSLMAFLGLAR

Nucleotide

• Length: 333 bp

>T197518 NZ_CP096231:c153301-152858 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA

Antitoxin

Protein

• Length: 52 a.a.
• Molecular weight: 5650.15 Da
• Isoelectric Point: 3.7453

>AT197518 WP_020171194.1 NZ_CP072203:c3752534-3752379 [Gordonia polyisoprenivorans]
MRPLQVWVPDVRTPEFAAQAHRQSVLVAEADADGDEQDFVEAVAAPWDDEA

Nucleotide

• Length: 156 bp

>AT197518 NZ_CP096231:c153528-153298 [Klebsiella pneumoniae subsp. pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA