Detailed information of TA system TA197514

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 85557..86092
• Replicon: plasmid unnamed2
• Accession: NZ_CP072202
• Organism: Klebsiella pneumoniae strain B5055

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: J6T75_RS27765
• Protein ID: WP_016531292.1
• Coordinates: 85557..85844 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: W9BAK2
• Locus tag: J6T75_RS27770
• Protein ID: WP_016531291.1
• Coordinates: 85841..86092 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J6T75_RS27745 (J6T75_27660)	81356..82576	-	1221	WP_032446666.1	ISL3-like element ISKox3 family transposase	-
J6T75_RS27750 (J6T75_27665)	82737..83213	+	477	WP_016529487.1	hypothetical protein	-
J6T75_RS27755 (J6T75_27670)	83470..83757	-	288	WP_016529486.1	hypothetical protein	-
J6T75_RS27760 (J6T75_27675)	83847..84509	-	663	WP_016529485.1	division plane positioning ATPase MipZ	-
J6T75_RS27765 (J6T75_27680)	85557..85844	-	288	WP_016531292.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
J6T75_RS27770 (J6T75_27685)	85841..86092	-	252	WP_016531291.1	toxin-antitoxin stability system antitoxin protein StbD	Antitoxin
J6T75_RS27775 (J6T75_27690)	86188..87459	-	1272	WP_046041953.1	Y-family DNA polymerase	-
J6T75_RS27780 (J6T75_27695)	87459..87899	-	441	WP_046041964.1	translesion error-prone DNA polymerase V autoproteolytic subunit	-
J6T75_RS27785 (J6T75_27700)	87939..89018	+	1080	WP_016531016.1	IS481 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	clbI / basG	1..95083	95083
	-	flank	IS/Tn	-	-	81356..82576	1220

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11120.16 Da
• Isoelectric Point: 10.4373

>T197514 WP_016531292.1 NZ_CP072202:c85844-85557 [Klebsiella pneumoniae]
MTYTVKFREDALKEWNKLDKTIQQQFAKKLKKCCENPHIPSAKLRGIKDCYKIKLRASGFRLVYQVIDNQLIIAIVAVGK
RERSDVYTLASERMK

Nucleotide

• Length: 288 bp

>T197514 NZ_CP096230:c4903648-4903364 [Klebsiella pneumoniae subsp. pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9285.68 Da
• Isoelectric Point: 4.2257

>AT197514 WP_016531291.1 NZ_CP072202:c86092-85841 [Klebsiella pneumoniae]
MPHIILSDTTASVSELKKNPMATVSAGDGFPVAILNRNQPAFYCVPAELYEKMLDALDDQELIKLVAERSNQPLFDMDLE
KYL

Nucleotide

• Length: 252 bp

>AT197514 NZ_CP096230:c4903880-4903638 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Antitoxin

• AlphaFold DB: W9BAK2