Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 69567..70210 Replicon plasmid unnamed2
Accession NZ_CP072202
Organism Klebsiella pneumoniae strain B5055

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag J6T75_RS27685 Protein ID WP_001044768.1
Coordinates 69567..69983 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag J6T75_RS27690 Protein ID WP_001261287.1
Coordinates 69980..70210 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J6T75_RS27650 (J6T75_27565) 64661..64915 - 255 WP_016528790.1 hypothetical protein -
J6T75_RS27655 (J6T75_27570) 64991..65248 - 258 WP_004098928.1 hypothetical protein -
J6T75_RS27660 (J6T75_27575) 65297..65500 - 204 WP_004150739.1 HHA domain-containing protein -
J6T75_RS27665 (J6T75_27580) 65561..66055 - 495 WP_016528789.1 hypothetical protein -
J6T75_RS27670 (J6T75_27585) 66086..66652 - 567 WP_009654312.1 hypothetical protein -
J6T75_RS27675 (J6T75_27590) 66649..66912 - 264 WP_009310051.1 hypothetical protein -
J6T75_RS27680 (J6T75_27595) 67267..69405 + 2139 WP_000350635.1 AAA family ATPase -
J6T75_RS27685 (J6T75_27600) 69567..69983 - 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
J6T75_RS27690 (J6T75_27605) 69980..70210 - 231 WP_001261287.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
J6T75_RS27695 (J6T75_27610) 70516..73635 + 3120 WP_032446663.1 hypothetical protein -
J6T75_RS27700 (J6T75_27615) 73898..75031 + 1134 WP_000545983.1 DUF3800 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - clbI / basG 1..95083 95083


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T197513 WP_001044768.1 NZ_CP072202:c69983-69567 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T197513 NZ_CP096230:c4741899-4741366 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCTGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT197513 WP_001261287.1 NZ_CP072202:c70210-69980 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT197513 NZ_CP096230:c4742176-4741910 [Klebsiella pneumoniae subsp. pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References