Detailed information of TA system TA197512

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 99649..100400
• Replicon: plasmid unnamed1
• Accession: NZ_CP072201
• Organism: Klebsiella pneumoniae strain B5055

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: W9BA31
• Locus tag: J6T75_RS26915
• Protein ID: WP_032104592.1
• Coordinates: 99649..100131 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: W9B1S8
• Locus tag: J6T75_RS26920
• Protein ID: WP_016529519.1
• Coordinates: 100122..100400 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J6T75_RS26890 (J6T75_26820)	95750..96202	+	453	WP_071829949.1	transcriptional repressor	-
J6T75_RS26895 (J6T75_26825)	96350..97578	+	1229	WP_115217266.1	IS3 family transposase	-
J6T75_RS26900 (J6T75_26830)	97963..98199	+	237	WP_016529523.1	hypothetical protein	-
J6T75_RS26905 (J6T75_26835)	98238..98870	-	633	WP_016529522.1	hypothetical protein	-
J6T75_RS26910 (J6T75_26840)	99207..99611	-	405	WP_016529521.1	DUF2251 domain-containing protein	-
J6T75_RS26915 (J6T75_26845)	99649..100131	-	483	WP_032104592.1	GNAT family N-acetyltransferase	Toxin
J6T75_RS26920 (J6T75_26850)	100122..100400	-	279	WP_016529519.1	DUF1778 domain-containing protein	Antitoxin
J6T75_RS26925 (J6T75_26855)	100708..101244	+	537	WP_032445791.1	hypothetical protein	-
J6T75_RS26930 (J6T75_26860)	101628..102638	-	1011	WP_004152284.1	zinc-binding alcohol dehydrogenase family protein	-
J6T75_RS26935	102981..103063	+	83	Protein_112	hypothetical protein	-
J6T75_RS26940 (J6T75_26865)	103099..104181	+	1083	WP_016528990.1	DNA-binding transcriptional repressor LacI	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	rmpA / iroN / iroD / iroC / iroB / iutA / iucD / iucC / iucB / iucA	1..164334	164334

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (44-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-92)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17781.73 Da
• Isoelectric Point: 9.4946

>T197512 WP_032104592.1 NZ_CP072201:c100131-99649 [Klebsiella pneumoniae]
MGMRPPEPLTPEHNIAEFCCQDQVLSEWLKKKALKNHRTGISRVFVVCAENTNRVIAYYCLASGSVHRNTVPGAYRRNAP
EALPVIVLGRLAVDAAWARKGLGAALLKDAIYRTEHIAIQVGVRALLVHALNDEVREFYTKFGFEPSIANALTLLFPIKT

Nucleotide

• Length: 483 bp

>T197512 NZ_CP096230:4150500-4150718 [Klebsiella pneumoniae subsp. pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10708.23 Da
• Isoelectric Point: 4.5156

>AT197512 WP_016529519.1 NZ_CP072201:c100400-100122 [Klebsiella pneumoniae]
MKPDIREAPINIRAKTSQRDLIDMAANLVSKSRTDFMLEVACREAQDILLDQRLFLLDDNQFNAFVEELDAPMTPERQAR
IDNLMSRKSPWE

Nucleotide

• Length: 279 bp

>AT197512 NZ_CP096230:4150099-4150473 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Toxin

• AlphaFold DB: W9BA31

Antitoxin

• AlphaFold DB: W9B1S8