Detailed information of TA system TA197511

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 27831..28741
• Replicon: plasmid unnamed1
• Accession: NZ_CP072201
• Organism: Klebsiella pneumoniae strain B5055

Toxin (Protein)

• Gene name: -
• Uniprot ID: W9B9V2
• Locus tag: J6T75_RS26515
• Protein ID: WP_016528981.1
• Coordinates: 27831..28301 (-)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: W9B1L8
• Locus tag: J6T75_RS26520
• Protein ID: WP_016528982.1
• Coordinates: 28298..28741 (-)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J6T75_RS26485 (J6T75_26425)	22939..23145	-	207	Protein_22	IS66 family transposase	-
J6T75_RS26490 (J6T75_26430)	23630..23788	+	159	WP_004181898.1	type I toxin-antitoxin system Hok family toxin	-
J6T75_RS26495 (J6T75_26435)	24429..24596	-	168	WP_004117900.1	hypothetical protein	-
J6T75_RS26500 (J6T75_26440)	25082..25861	+	780	WP_225932725.1	Hok/Gef family protein	-
J6T75_RS26510 (J6T75_26450)	27460..27720	+	261	WP_016528980.1	hypothetical protein	-
J6T75_RS26515 (J6T75_26455)	27831..28301	-	471	WP_016528981.1	RES family NAD+ phosphorylase	Toxin
J6T75_RS26520 (J6T75_26460)	28298..28741	-	444	WP_016528982.1	DUF2384 domain-containing protein	Antitoxin
J6T75_RS26525 (J6T75_26465)	28842..29331	-	490	Protein_30	DUF4113 domain-containing protein	-
J6T75_RS26530 (J6T75_26470)	29440..29661	-	222	WP_016528984.1	DUF2188 domain-containing protein	-
J6T75_RS26535 (J6T75_26475)	29842..29961	-	120	Protein_32	Fe3+-citrate ABC transporter substrate-binding protein	-
J6T75_RS26540 (J6T75_26480)	30036..32162	-	2127	WP_032105434.1	TonB-dependent Fe(3+) dicitrate receptor FecA	-
J6T75_RS26545 (J6T75_26485)	32249..33202	-	954	Protein_34	fec operon regulator FecR	-
J6T75_RS26550 (J6T75_26490)	33199..33720	-	522	WP_004213635.1	ferric citrate uptake sigma factor FecI	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	rmpA / iroN / iroD / iroC / iroB / iutA / iucD / iucC / iucB / iucA	1..164334	164334

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre/MbcA/ParS-like, toxin-binding domain (94-144)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17603.85 Da
• Isoelectric Point: 4.4093

>T197511 WP_016528981.1 NZ_CP072201:c28301-27831 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPDNWADEDAPQELADYGDAWSFTWSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

>T197511 NZ_CP096230:c2716164-2714842 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGCGACGCTTACCACCTGGATGAACAATGTGCGCGTGGGAACCTTAACCCGCCAGGCTAACGGGGCGCACAGTTTTCG
CTATGAGGAAGAGTGGCTGCGCAGCCCGCGCGCCAGACCGCTCTCTCTCTCACTGCCGCTGCAGTATGGCAATATCACGG
CCGACGCGGTGTATCACTATTTTGATAACCTGCTGCCCGACAGCCCGCAGGTCCGCGACCGGATCGTCAGGCGCTATCAA
GCGCGCTCGAAGCAGCCGTTCGATCTGCTGGCCGAAGTCGGGCGGGATAGCGTAGGGGCGGTTACCCTCCTGCCGCCGGG
GGAAGAGGCGCACCTGGAAGGATTACGCTGGCAGACGCTGGACGAGGCGCAGCTCACCGCTTTGCTGACCGCTTACCAGT
CGGATATCCCCCTCGGGATGATTACCGGCCAGGACGATTTCCGTATCTCGGTGGCCGGCGCCCAGGAGAAAACGGCGCTG
CTGCGGATGGGCGAGCAGTGGTGTATTCCGCAGGGCGCCACGCCGACCACGCATATCATTAAGCTGCCCATCGGCGAGAT
CAAGCAGCCCAATGCGACGCTGGATCTGCGTGAAAGCGTGGATAATGAGTTTCTGTGCCTGGCGCTGGCCAGAGAACTGG
GCCTGGCGGTGCCGGAAGCGGAGATCATCACCACCCCGCGGATCCGCGCCCTGGCGGTAACCCGCTTCGACCGGCGCTGG
GCGCAGGAGGGACGGGTGCTGCTGCGTCTGCCGCAGGAGGATCTGTGTCAGGCTTTTGGTCTTCCTTCAGCGATGAAATA
TGAGTCTGACGGCGGGCCGGGTATCGCCGCGATCATGACCTTTCTGCTGGGCTCGAGTGAAGCGCTGAAAGATCGCTATG
ACTTTATGAAGTTCATGGTCTTCCAGTGGCTGACGGGGGCGACGGACGGTCACGCAAAAAACTTCTCAATTTACCTGTTG
CCCGGCGGCAGCTACCGCTTAACACCGTTCTACGACATTATTTCGGCGTTTCCGGTGCTGGGCGGCACGGGACTGCATCT
GCGCGATCTGAAGCTGTCGATGGGGCTCAATGCCACAAAAGGGCGTAAAACAGAGATTAACGCCATCTATCCGCGTCATT
TTCTGGCGACGGCCAAAGCGGTGAACTTTCCCCGGGAGCAGATGCTGGCGATCCTCCAGGAGTTTGCCGGGCATGTGCCG
CAGGCAATCGAAAGCGCTCGCCGGACGCTGCCGGCGGACTTTTCCAGCCACGTCTGGCAGGCCATTACAGAGAACATGCT
GAAGCTGCACGCCCGCCTGCAGCAAGGGCTGCAGGCGCTATAG

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16494.83 Da
• Isoelectric Point: 10.2498

>AT197511 WP_016528982.1 NZ_CP072201:c28741-28298 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAAQKWLNEPNRALSWKVPADLMASETGAYEIIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT197511 NZ_CP096230:c2716430-2716164 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAATTCCCCGCAGATATACAGTCCCGTGCAGCTGGCCAATTACCTGAAGCTGCTGCGCCAGCAGAACCACTGGACCCA
GGATGCGCTGGCCAGGCGGATCGGCATTAAGCAGGCCACGCTGTCCAATTTTGAAAATCACCCCGACAAAACCACCTTAA
CCACGCTGTTTAAGATCCTCCAGTCGCTGGGGGTGGTGATGACGCTGAACGCCAAAGCTGCTGAGACGGCGCCGGCTCCC
GATAACAACACGGACCTTGACTGGTAA

Structures

Toxin

• AlphaFold DB: W9B9V2

Antitoxin

• AlphaFold DB: W9B1L8