Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 4987422..4988047 Replicon chromosome
Accession NZ_CP072200
Organism Klebsiella pneumoniae strain B5055

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag J6T75_RS24195 Protein ID WP_002882817.1
Coordinates 4987422..4987805 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag J6T75_RS24200 Protein ID WP_004150355.1
Coordinates 4987805..4988047 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J6T75_RS24180 (J6T75_24130) 4984788..4985690 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
J6T75_RS24185 (J6T75_24135) 4985687..4986322 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
J6T75_RS24190 (J6T75_24140) 4986319..4987248 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
J6T75_RS24195 (J6T75_24145) 4987422..4987805 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
J6T75_RS24200 (J6T75_24150) 4987805..4988047 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
J6T75_RS24205 (J6T75_24155) 4988249..4989166 + 918 WP_004178029.1 alpha/beta hydrolase -
J6T75_RS24210 (J6T75_24160) 4989180..4990121 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
J6T75_RS24215 (J6T75_24165) 4990166..4990603 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
J6T75_RS24220 (J6T75_24170) 4990600..4991460 - 861 WP_002882807.1 virulence factor BrkB family protein -
J6T75_RS24225 (J6T75_24175) 4991454..4992053 - 600 WP_046043904.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T197507 WP_002882817.1 NZ_CP072200:c4987805-4987422 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T197507 NZ_CP096230:326550-326918 [Klebsiella pneumoniae subsp. pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACTGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT197507 WP_004150355.1 NZ_CP072200:c4988047-4987805 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT197507 NZ_CP096230:326332-326553 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References