Detailed information of TA system TA197498

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2546173..2547761
• Replicon: chromosome
• Accession: NZ_CP072200
• Organism: Klebsiella pneumoniae strain B5055

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: W9B834
• Locus tag: J6T75_RS12705
• Protein ID: WP_016529318.1
• Coordinates: 2546173..2547495 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: J6T75_RS12710
• Protein ID: WP_004176198.1
• Coordinates: 2547495..2547761 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J6T75_RS12690 (J6T75_12640)	2541229..2543730	+	2502	Protein_2483	fimbrial biogenesis outer membrane usher protein	-
J6T75_RS12695 (J6T75_12645)	2543718..2544722	+	1005	WP_009308876.1	fimbrial protein	-
J6T75_RS12700 (J6T75_12650)	2544864..2546156	+	1293	WP_002904794.1	glycoside hydrolase family 10 protein	-
J6T75_RS12705 (J6T75_12655)	2546173..2547495	-	1323	WP_016529318.1	type II toxin-antitoxin system HipA family toxin	Toxin
J6T75_RS12710 (J6T75_12660)	2547495..2547761	-	267	WP_004176198.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
J6T75_RS12715 (J6T75_12665)	2548054..2548380	-	327	WP_002904788.1	hydroxyisourate hydrolase	-
J6T75_RS12720 (J6T75_12670)	2548377..2548877	-	501	WP_046043283.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
J6T75_RS12725 (J6T75_12675)	2548855..2550234	-	1380	WP_004176204.1	nucleobase:cation symporter-2 family protein	-
J6T75_RS12730 (J6T75_12680)	2550580..2551734	+	1155	WP_046043281.1	FAD-dependent urate hydroxylase HpxO	-
J6T75_RS12735 (J6T75_12685)	2551715..2552638	-	924	WP_016529218.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	mgtC / iroE	2365645..2582632	216987

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (5-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (13-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49125.44 Da
• Isoelectric Point: 7.5366

>T197498 WP_016529318.1 NZ_CP072200:c2547495-2546173 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITGQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEFLCLALARELGLAVPEAEIITTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T197498 NZ_CP096227:99686-99964 [Xanthomonas citri pv. citri]
GTGCCGGCATTGGCTGATCTGGACGCAATTGCCGACTACATCGCAATCGATAACGCACCCGCTGCAGCGGCGCTGGTGAA
ACGGGTGTTCGGGCACGTTGAGCAATTGATCGAACACCCGGACAGCGGAAGCCGACCACAGGAACTCAAGCGCTCGCGCT
ATCGCCAGATTGTCGAACCACCTTGCCGCGTGTTCTACCGCGTGGATGGGCAGCGCATCGTGGTGGTGCACGTCATGCGG
TCGGAGCGCGCGCTTCGCGGGAACCGTCTTTCACGCTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9936.36 Da
• Isoelectric Point: 8.5912

>AT197498 WP_004176198.1 NZ_CP072200:c2547761-2547495 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMMLNAKAAETAPAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT197498 NZ_CP096227:99420-99677 [Xanthomonas citri pv. citri]
ATGCGCACCGAACTTGTCACCACGCTCAAGCGTCAGGCGACCGAACTCCTTGCAGCTGCAGAGCGCGACAAGGAGCCAAT
CCTGATCACACAGCACGGTTTACCCAGTGCCTATCTGGTGGATGTTGCCAGCTATGAGCGCATGCAGCAGCGCATTGCGC
TCCTCGAAGGAATCGCCCGTGGAGAAATGGCAGTCGCGGAAGGCCGTACGCTGAGCCATGAACAGGCACGGCACCGCATG
GCGCGATGGCTGAAATAA

Structures

Toxin

• AlphaFold DB: W9B834