Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1457355..1457974 Replicon chromosome
Accession NZ_CP072200
Organism Klebsiella pneumoniae strain B5055

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag J6T75_RS07245 Protein ID WP_002892050.1
Coordinates 1457355..1457573 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag J6T75_RS07250 Protein ID WP_002892066.1
Coordinates 1457600..1457974 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J6T75_RS07210 (J6T75_07200) 1453402..1453665 + 264 WP_002892011.1 type B 50S ribosomal protein L31 -
J6T75_RS07215 (J6T75_07205) 1453665..1453805 + 141 WP_002892018.1 type B 50S ribosomal protein L36 -
J6T75_RS07220 (J6T75_07210) 1453802..1454500 - 699 WP_002892021.1 GNAT family protein -
J6T75_RS07225 (J6T75_07215) 1454601..1456052 + 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
J6T75_RS07230 (J6T75_07220) 1456027..1456497 - 471 WP_002892026.1 YlaC family protein -
J6T75_RS07235 (J6T75_07225) 1456518..1456658 + 141 WP_004147370.1 hypothetical protein -
J6T75_RS07240 (J6T75_07230) 1456630..1457196 - 567 WP_002892030.1 maltose O-acetyltransferase -
J6T75_RS07245 (J6T75_07235) 1457355..1457573 - 219 WP_002892050.1 HHA domain-containing protein Toxin
J6T75_RS07250 (J6T75_07240) 1457600..1457974 - 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
J6T75_RS07255 (J6T75_07245) 1458460..1461606 - 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
J6T75_RS07260 (J6T75_07250) 1461629..1462822 - 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T197497 WP_002892050.1 NZ_CP072200:c1457573-1457355 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T197497 NZ_CP096221:c40261-39911 [Enterococcus faecium]
ATGGTGAGAAAACCTAGACAAGGCGATATTCTATTATTGAATACAGCACCTCGATCTGGACACGAACAGACAGGTAAAAG
ACCTTATATTGTTTTAAGTCATGATATTATTGCGGATTATAGTAACGTTGTTACAGTTGCTCCAATTTCTAGTACAACTA
GAAACTATCCGCTATATGTAAATATAAACTCAGAATATGAAATGAAGACAACAGGAAAAGTATTATTAGATCAACTAACG
ACAATTGATTATGAAGCCAGAGAGTGTATTTTTTTGGAAAAAGCGCATGATAGTTTAGTTGAGGAGTTATTGATGAAAGT
TAGAACAGTTTTTCAAAAAGTAAGCAAATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT197497 WP_002892066.1 NZ_CP072200:c1457974-1457600 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT197497 NZ_CP096221:c40518-40255 [Enterococcus faecium]
ATGGAAATGTTACCTATAAAAAAATGGGGAAATAGTAATGGCCTTAGATTGCCAAAGTATATTATGGAATATCTTGGAAT
TCATACAGAAGACAAAGTGAAGATAATACAAGAAGAAGTAAACGGTCAGAAGCGGCTAATTATCGAAGCGGCTAATATAG
AAAATGAATTAACAATTGAACAGTTATTTGAAAATTACACTGATGAAAGAAATCATGTGACGATTCAAAATTTAGGTGAA
GCAGTAGGTAATGAAAAATGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References