Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 3256634..3257181 Replicon chromosome
Accession NZ_CP072199
Organism Serratia marcescens subsp. marcescens ATCC 13880

Toxin (Protein)


Gene name ccdB Uniprot ID A0A380ADA6
Locus tag J6N75_RS15565 Protein ID WP_025302144.1
Coordinates 3256873..3257181 (+) Length 103 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A0G3SUU9
Locus tag J6N75_RS15560 Protein ID WP_033633717.1
Coordinates 3256634..3256870 (+) Length 79 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J6N75_RS15545 (J6N75_15545) 3253120..3254655 + 1536 WP_033640885.1 glutathione ABC transporter substrate-binding protein GsiB -
J6N75_RS15550 (J6N75_15550) 3254708..3255628 + 921 WP_016928458.1 glutathione ABC transporter permease GsiC -
J6N75_RS15555 (J6N75_15555) 3255638..3256546 + 909 WP_181817231.1 glutathione ABC transporter permease GsiD -
J6N75_RS15560 (J6N75_15560) 3256634..3256870 + 237 WP_033633717.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
J6N75_RS15565 (J6N75_15565) 3256873..3257181 + 309 WP_025302144.1 CcdB family protein Toxin
J6N75_RS15570 (J6N75_15570) 3257218..3258060 - 843 WP_015377214.1 S-formylglutathione hydrolase -
J6N75_RS15575 (J6N75_15575) 3258075..3259199 - 1125 WP_016928454.1 S-(hydroxymethyl)glutathione dehydrogenase/class III alcohol dehydrogenase -
J6N75_RS15580 (J6N75_15580) 3259229..3260149 - 921 WP_033640884.1 LysR family transcriptional regulator -
J6N75_RS15585 (J6N75_15585) 3260255..3261412 + 1158 WP_033640883.1 YbfB/YjiJ family MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-101)

Antitoxin

Post-segregation antitoxin CcdA

(8-78)


Sequences

Toxin        


Download         Length: 103 a.a.        Molecular weight: 11504.32 Da        Isoelectric Point: 5.0196

>T197480 WP_025302144.1 NZ_CP072199:3256873-3257181 [Serratia marcescens subsp. marcescens ATCC 13880]
MQFTVYDNTYPASAYPYLLDVQSDLIDVLSTRLMIPLYALDNVRVKISARLCPEIEVNGEKFLVMTHEMAAVRISQIGKA
VGNVNEHRNQIKAAIDFLIDGF

Download         Length: 309 bp

>T197480 NZ_CP072199:3256873-3257181 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGCAATTTACCGTATATGACAATACGTATCCGGCATCAGCCTATCCCTACTTACTGGACGTTCAAAGCGATCTTATTGA
TGTCCTCTCGACCCGGTTAATGATCCCTCTTTATGCTTTGGATAATGTCAGGGTTAAAATTTCTGCACGTTTATGCCCGG
AGATTGAGGTCAACGGCGAAAAGTTTCTTGTTATGACGCATGAGATGGCTGCCGTACGTATTTCGCAGATTGGGAAGGCC
GTTGGTAATGTCAATGAGCACAGGAATCAAATTAAAGCCGCCATCGATTTTCTGATTGATGGTTTTTGA

Antitoxin


Download         Length: 79 a.a.        Molecular weight: 8994.07 Da        Isoelectric Point: 5.0807

>AT197480 WP_033633717.1 NZ_CP072199:3256634-3256870 [Serratia marcescens subsp. marcescens ATCC 13880]
MANNNVTKKTVNVTIDRDLFQRAKSLGVNVSSVLTDALSVRVRDIEIQQWREQNRAALEELNRITEENGLLSDEYRTF

Download         Length: 237 bp

>AT197480 NZ_CP072199:3256634-3256870 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGGCAAATAACAATGTGACGAAAAAGACAGTCAACGTAACCATCGATCGCGATCTTTTCCAGCGCGCAAAATCCCTGGG
CGTCAATGTGTCATCTGTATTGACGGACGCATTGAGCGTCCGTGTCAGGGACATTGAGATTCAACAGTGGCGCGAGCAAA
ATCGTGCGGCGTTAGAAGAACTTAATCGTATCACTGAAGAAAATGGTTTGTTGTCTGATGAATATCGGACATTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A380ADA6

Antitoxin

Source ID Structure
AlphaFold DB A0A0G3SUU9

References