Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 2678434..2679085 Replicon chromosome
Accession NZ_CP072181
Organism Lacticaseibacillus paracasei strain HL182

Toxin (Protein)


Gene name pemK Uniprot ID K6S8L5
Locus tag J3H89_RS12895 Protein ID WP_003567661.1
Coordinates 2678434..2678817 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID K6RKI5
Locus tag J3H89_RS12900 Protein ID WP_003567665.1
Coordinates 2678837..2679085 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J3H89_RS12890 (J3H89_002578) 2677729..2678250 - 522 WP_003567657.1 QueT transporter family protein -
J3H89_RS12895 (J3H89_002579) 2678434..2678817 - 384 WP_003567661.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
J3H89_RS12900 (J3H89_002580) 2678837..2679085 - 249 WP_003567665.1 antitoxin Antitoxin
J3H89_RS12905 (J3H89_002581) 2679153..2680289 - 1137 WP_208841542.1 alanine racemase -
J3H89_RS12910 (J3H89_002582) 2680276..2680650 - 375 WP_003567669.1 holo-ACP synthase -
J3H89_RS12915 (J3H89_002583) 2680819..2682327 - 1509 WP_003567670.1 DEAD/DEAH box helicase -
J3H89_RS12920 (J3H89_002584) 2682453..2682596 - 144 WP_016380391.1 hypothetical protein -
J3H89_RS12925 (J3H89_002585) 2682627..2684015 - 1389 WP_003581096.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-112)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 13773.03 Da        Isoelectric Point: 10.9764

>T197472 WP_003567661.1 NZ_CP072181:c2678817-2678434 [Lacticaseibacillus paracasei]
MDVSVKRGDVFFADLSPVVGSEQGGNRPVLIIQNNVGNHYSPTVIVAAITSKIQKPKMPTHVGLRAKQDGVERNSVILLE
QIRTIDKQRLQARVTALSSTKMAAVDRALAISVGLVSLPKPKTYNKN

Download         Length: 384 bp

>T197472 NZ_CP096201:3240764-3241141 [Kluyvera ascorbata]
TTGCAAATCCTTTCTGCGCCACCGGCGCGGGCGGCATTGTCACGCCCATCCCCTGTAGCTGTCTGGCAAATACTGCTGTC
ACATCTGCTGGCACAACACTACGGCCTGACCCTGAATGACACCCCGTTCAGCGATGAAACCATTATCCGCAAGCATATCG
ATGCAGGTATCTCACTCAGCGATGCGGTCAATTTTATCGTCGAAAAATATGAGCTGGTACGGACTGACCGTCACGGTTCT
ACAGTGGCGGAACAGTCACCTTTTATCAGCAGCATCGACATGTTCCACGCCCGCCGGGCTACCGGGCTGATGAAACGACG
GGGTTACAAAACCATCAGCAATATCATCACGGGTGTTTATCGACAGGAGCAACCATGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 8938.08 Da        Isoelectric Point: 4.4610

>AT197472 WP_003567665.1 NZ_CP072181:c2679085-2678837 [Lacticaseibacillus paracasei]
MKKQTSVAVTFDDAIKARLDAYCELNDIDAQAVITEAVATFLKSQAPEINQLISGYVAMGQINTEISHAFSACESEAYAH
IR

Download         Length: 249 bp

>AT197472 NZ_CP096201:3240362-3240715 [Kluyvera ascorbata]
ATGAAAAAAGACACCGTCAACAAAACCACAGATACGCTGCAATGGGGACTGAAATGCGACATCACGCCGCATTTCGGCGC
ACGTCTGGTGCTGGAAGGCAACCGCCTGCATTACCTGGCTGACCGGGCCAGTATCACAGGGGCATTCAGTGAGGCAGATT
TACGCCATCTCGACCAGGCTTTTCCGGTGCTGCTGAAGCAACTGGAACTGATGCTGACCAGCGGTGAGCTTAATCCCCGT
CACCAGCACACCGTTACCCTGTATGCGAAAGGGCTGACCTGCGAGGCCGACACTCTGGGCAGTTGCGGTTATGTTTACCT
CGCTATTTACCCGACATTCACAACAGCAACGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB K6S8L5

Antitoxin

Source ID Structure
AlphaFold DB A0A0E2BNY2

References