Detailed information of TA system TA197469

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RelB
• Location: 20802..21400
• Replicon: plasmid pTiAB2_73
• Accession: NZ_CP072175
• Organism: Rhizobium sp. AB2/73

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: J5284_RS30985
• Protein ID: WP_209159983.1
• Coordinates: 20802..21095 (-)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: J5284_RS30990
• Protein ID: WP_209159982.1
• Coordinates: 21092..21400 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J5284_RS30945 (J5284_30945)	16533..16672	-	140	Protein_16	fasciclin domain-containing protein	-
J5284_RS30950 (J5284_30950)	16694..17107	-	414	WP_209159985.1	DoxX family protein	-
J5284_RS30955 (J5284_30955)	17091..18056	-	966	WP_257714020.1	alpha/beta hydrolase	-
J5284_RS30960 (J5284_30960)	18099..18272	-	174	WP_219511847.1	hypothetical protein	-
J5284_RS30965 (J5284_30965)	18262..18402	-	141	WP_219511825.1	hypothetical protein	-
J5284_RS30970 (J5284_30970)	18984..19451	-	468	Protein_21	class I SAM-dependent methyltransferase	-
J5284_RS30975 (J5284_30975)	19496..20149	-	654	WP_229002292.1	ABC transporter permease	-
J5284_RS35225	20176..20378	+	203	Protein_23	siderophore-interacting protein	-
J5284_RS35230	20484..20741	+	258	WP_255610431.1	SIP domain-containing protein	-
J5284_RS30985 (J5284_30985)	20802..21095	-	294	WP_209159983.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
J5284_RS30990 (J5284_30990)	21092..21400	-	309	WP_209159982.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
J5284_RS30995 (J5284_30995)	21671..22117	+	447	WP_209159981.1	DNA-binding protein	-
J5284_RS31000 (J5284_31000)	22107..22685	+	579	WP_209159980.1	PIN domain-containing protein	-
J5284_RS31005 (J5284_31005)	22835..25195	+	2361	WP_209159979.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..605541	605541

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (2-90)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (4-52)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 10568.08 Da
• Isoelectric Point: 5.7904

>T197469 WP_209159983.1 NZ_CP072175:c21095-20802 [Rhizobium sp. AB2/73]
VKLTWSAFALSDRDAIFTYIEADNPAAAVLVDERIVAASRRLLDFPASGRVGRIAGTRELVINGTPYVAVYAVTDTTVRI
LRVLHGAQEWPESVPIS

Nucleotide

• Length: 294 bp

>T197469 NZ_CP096201:1371079-1371483 [Kluyvera ascorbata]
ATGCTCAAGTATATGCTGGATACTAATATTTGTATCTATACCATCAAGAATAAACCGCAGGCGGTTCGTGAAGCATTCAC
TCAGCACTACGGGCGAATGTGCATCAGTTCCGTCACGTTGATGGAGTTAATCTATGGGGCGGAAAAATCCGCAGCGCCGG
AAAAAAATCTGCGCGTGGTAGAAGGTTTTGTGGCCCGGCTGGAGGTACTGAACTATGGCAATGACGCCGCCATTCAGACC
GGGCAGATAAGGGCTGAGTTGGCAAAGGCAGGCACCCCTATTGGCCCGTATGACAGTATGATAGCCGCCCATGCTCGTTC
GCTCGGGTTGATTATGGTCACTAATAATACCCGCGAATTTGAGCGAATCAGCGGGCTGCGTCTCGAAGACTGGAGCATTA
GCTAG

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11055.46 Da
• Isoelectric Point: 4.8892

>AT197469 WP_209159982.1 NZ_CP072175:c21400-21092 [Rhizobium sp. AB2/73]
MAANALVQTRIDAEVRDRASAVLESMGLTVSDAVRILLTRTANEGALPLELLSGSEAHDVWFRNKVLEALNDTRPDVSDD
EVEAHFAERRAAARLKAGALKS

Nucleotide

• Length: 309 bp

>AT197469 NZ_CP096201:1370849-1371079 [Kluyvera ascorbata]
ATGGAAAAGACAACGATATTCAAAAGCAACCGCAGCCAGGCGGTGCGTTTGCCAAAAGCGGTAGCGATGCCGGAAGACGT
TAAACACGTCGATATTATTGCTATTGGGCGGACACGAGTGATTACGCCTGCAGGTGAAAGCTGGGATAGCTGGTTTGACG
GCGAAAGCGTTACATCAGATTTTATGGCGACGCGGGAACAGCCGGGTGAACAGGATAGGGAAACGTTCTGA