Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 3853067..3853677 Replicon chromosome
Accession NZ_CP072172
Organism Rhizobium sp. AB2/73

Toxin (Protein)


Gene name hicA Uniprot ID A0A1E4Y882
Locus tag J5284_RS18935 Protein ID WP_069613844.1
Coordinates 3853067..3853321 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A1E4Y890
Locus tag J5284_RS18940 Protein ID WP_069613843.1
Coordinates 3853318..3853677 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J5284_RS18915 (J5284_18910) 3848141..3849010 - 870 WP_069613848.1 hydroxymethylglutaryl-CoA lyase -
J5284_RS18920 (J5284_18915) 3849007..3850995 - 1989 WP_069613847.1 acetyl/propionyl/methylcrotonyl-CoA carboxylase subunit alpha -
J5284_RS18925 (J5284_18920) 3851005..3851388 - 384 WP_069613846.1 RidA family protein -
J5284_RS18930 (J5284_18925) 3851399..3853006 - 1608 WP_069613845.1 carboxyl transferase domain-containing protein -
J5284_RS18935 (J5284_18930) 3853067..3853321 + 255 WP_069613844.1 type II toxin-antitoxin system HicA family toxin Toxin
J5284_RS18940 (J5284_18935) 3853318..3853677 + 360 WP_069613843.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
J5284_RS18945 (J5284_18940) 3853717..3854880 - 1164 WP_069613842.1 isovaleryl-CoA dehydrogenase -
J5284_RS18950 (J5284_18945) 3854967..3858440 - 3474 WP_069613841.1 indolepyruvate ferredoxin oxidoreductase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(23-81)

Antitoxin

Uncharacterised protein family HicB

(52-102)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 9541.08 Da        Isoelectric Point: 10.5590

>T197464 WP_069613844.1 NZ_CP072172:3853067-3853321 [Rhizobium sp. AB2/73]
MNSKQRQTLEAIFSNPVKATIKWQDIEALLVAIGCEMSEGSGSRVRFTLSGRTLFIHRPHPKPDTKQYVVRTVREFLTDL
GIKP

Download         Length: 255 bp

>T197464 NZ_CP096201:320721-321089 [Kluyvera ascorbata]
ATGAACCTTCAGATTATCTCAGCGGAAGAGATAATAGCGTTTCATGACAGGCTGCTCCGCGTGACGCCTGGTGTTGTGGG
CATGCCCGATCCCGGACGTGCGGAAGCGTTAATGTATCGGGTGCTTAACCGGATCGAATATGAGGGTGTGACTGACGTGT
GGCTACTGGCAGCAATGCATCTGCTGGCCATATCGCGGGGGCATATTTTCAATGATGGTAATAAGCGCACCGCGCTGTTT
ATCACGCTGCTGTTTTTAAAGCGCAATGGTATTACACTCGCGGCCAATCCGGATTATGTGGCGATGACGGTTGAGGCTGC
TGCGGGCCAGTTGACGCTGGAGCAAATAGCCTCCCGCTTGCGTGGATAA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13335.86 Da        Isoelectric Point: 4.7352

>AT197464 WP_069613843.1 NZ_CP072172:3853318-3853677 [Rhizobium sp. AB2/73]
MRSYKGYTGSVEFDEDDMVFHGRIVGIRDIVTFEAETAQELVQAFRDSVDDYLAFCAKRSQEPDRPFSGNLSLRMTPRLH
EQVVRAAGNKAQSVNQWITDTLTEAASRDAESASMVVKA

Download         Length: 360 bp

>AT197464 NZ_CP096201:320503-320724 [Kluyvera ascorbata]
ATGCGTACGATTAACTATAGCGAGGCACGACAGAATCTAGCCGAAGTGCTGGAGAGCGCTGTGACGGGCGTACCTGTCAC
CATCACACGTCGTGGGCATAAACCTGCGGTCATCATTAGCGCTGAAGAGTTTGAGCGCTATCAGGCAGCCAGACTCGACG
ATGAATTTGCGGCCATCATGGCGATTCACGGCAACGAGATTGAGGAGTTGGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1E4Y882

Antitoxin

Source ID Structure
AlphaFold DB A0A1E4Y890

References