Detailed information of TA system TA197463

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 3737615..3738174
• Replicon: chromosome
• Accession: NZ_CP072172
• Organism: Rhizobium sp. AB2/73

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: J5284_RS18365
• Protein ID: WP_112724384.1
• Coordinates: 3737809..3738174 (+)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A1E4Y0V5
• Locus tag: J5284_RS18360
• Protein ID: WP_069616104.1
• Coordinates: 3737615..3737812 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J5284_RS18335 (J5284_18330)	3732675..3733079	-	405	WP_069616108.1	helix-turn-helix domain-containing protein	-
J5284_RS18340 (J5284_18335)	3733185..3734198	+	1014	WP_113366344.1	zinc-binding alcohol dehydrogenase family protein	-
J5284_RS18345 (J5284_18340)	3734553..3735926	+	1374	WP_069616106.1	magnesium transporter	-
J5284_RS18350 (J5284_18345)	3735992..3736465	-	474	WP_069616105.1	Lrp/AsnC family transcriptional regulator	-
J5284_RS18355 (J5284_18350)	3736610..3737512	+	903	WP_069616311.1	DMT family transporter	-
J5284_RS18360 (J5284_18355)	3737615..3737812	+	198	WP_069616104.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
J5284_RS18365 (J5284_18360)	3737809..3738174	+	366	WP_112724384.1	type II toxin-antitoxin system VapC family toxin	Toxin
J5284_RS18370 (J5284_18365)	3738176..3738703	-	528	WP_083258374.1	GNAT family N-acetyltransferase	-
J5284_RS18375 (J5284_18370)	3738710..3740374	-	1665	WP_069616103.1	electron transfer flavoprotein-ubiquinone oxidoreductase	-
J5284_RS18380 (J5284_18375)	3740663..3741451	+	789	WP_069616102.1	SDR family oxidoreductase	-
J5284_RS18385 (J5284_18380)	3741872..3743104	+	1233	WP_069616101.1	efflux RND transporter periplasmic adaptor subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-113)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-47)

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13559.68 Da
• Isoelectric Point: 7.4003

>T197463 WP_112724384.1 NZ_CP072172:3737809-3738174 [Rhizobium sp. AB2/73]
MILVDTSIWIDHFHTPEARLQSLLEQKQVVVHPFVVGEISLGNLRQYDLVIGSLRRLPAIARASDEDVLYFIRSNKLQGT
GIGYIDAHLAAATILTPGTYLWTRDRRLQRVAENLGIAAPL

Nucleotide

• Length: 366 bp

>T197463 NZ_CP096201:51653-51994 [Kluyvera ascorbata]
ATGTGGTCGGTGGAAACAACGGATGTTTTCGACAGTTGGTTTGAGGAACAGGCCGAAGCCTTGAGGGAAGATGTGCTTGC
AGCGATGGTGATTTTATCCGAGTACGGACCGCAGTTGTCGCGCCCGTTTGCGGATACGGTTAATGGCTCCGCTTTTTCAA
ATATGAAAGAGCTTCGTATACAGCATCAGGGAAATCCCATTCGGGCCTTTTTTGCGTTTGATCCTTCTCGCTGCGCCATC
GTGCTATGTGCAGGAGATAAAACAGGATTAAACGAAAGACGGTTTTATAAAGAGATGATCAAACTTGCTGACGCCGAGTA
CCGTAAGCACCTGAATAAGTGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 7045.13 Da
• Isoelectric Point: 6.2935

>AT197463 WP_069616104.1 NZ_CP072172:3737615-3737812 [Rhizobium sp. AB2/73]
MRTTVAIDDKLLADAREVTGLAETSAILREALKALVEREAAKTLIRLGGTEPNITAPPRRQFGVE

Nucleotide

• Length: 198 bp

>AT197463 NZ_CP096201:52004-52303 [Kluyvera ascorbata]
ATGGCGACCCTTAAAGAGCTAATGGCTAAACAGAGCCCGGAAAGTCAGGCGCGAATTGCTGAAAAAGTCGAAGCGTTACG
CCAGACCGTCACGTTAAGCATGTTGCGTGAAGAGCTCAACCTGTCTCAGGCGGAGTTGGCTGCCGCAATGGGCGTAAAAC
AGCCCACCGTGGCGAAGATGGAGCAGGCGGAAAACGATCCTCGCTTGTCGACACTGAAGCGTTACGTCGCTGCACTCGGC
GGTGAGATCAGCATTGATGTCACGCTGCCAACGGGAAAGAAGATAGCGTTTCATCTTTAA

Structures

Antitoxin

• AlphaFold DB: A0A1E4Y0V5