Detailed information of TA system TA197457

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 73988..74468
• Replicon: chromosome
• Accession: NZ_CP072172
• Organism: Rhizobium sp. AB2/73

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: -
• Locus tag: J5284_RS00375
• Protein ID: WP_083258161.1
• Coordinates: 74199..74468 (+)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: A0A1E4YCA6
• Locus tag: J5284_RS00370
• Protein ID: WP_069612387.1
• Coordinates: 73988..74215 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J5284_RS00350 (J5284_00350)	69891..70340	+	450	WP_069612391.1	DoxX family protein	-
J5284_RS00355 (J5284_00355)	70417..71112	-	696	WP_069612390.1	ATP phosphoribosyltransferase	-
J5284_RS00360 (J5284_00360)	71112..72230	-	1119	WP_069612389.1	ATP phosphoribosyltransferase regulatory subunit	-
J5284_RS00365 (J5284_00365)	72291..73814	-	1524	WP_069612388.1	histidine--tRNA ligase	-
J5284_RS00370 (J5284_00370)	73988..74215	+	228	WP_069612387.1	DUF6290 family protein	Antitoxin
J5284_RS00375 (J5284_00375)	74199..74468	+	270	WP_083258161.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
J5284_RS00380 (J5284_00380)	74544..74921	+	378	WP_069612386.1	hypothetical protein	-
J5284_RS00385 (J5284_00385)	74995..75363	+	369	WP_069612385.1	DUF305 domain-containing protein	-
J5284_RS00390 (J5284_00390)	75411..76310	-	900	WP_069612384.1	LysR family transcriptional regulator	-
J5284_RS00395 (J5284_00395)	76421..77446	+	1026	WP_069612383.1	NAD(P)-dependent alcohol dehydrogenase	-
J5284_RS00400 (J5284_00400)	77495..78544	-	1050	WP_069612382.1	aldo/keto reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-88)

Antitoxin

• Relaxosome protein TraY (7-50)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10621.19 Da
• Isoelectric Point: 10.3321

>T197457 WP_083258161.1 NZ_CP072172:74199-74468 [Rhizobium sp. AB2/73]
VAWTIEYARSARKFVEKLNPETRKRIRSFLEERLATLHDPRQVGDALQGARLGNYWRYRVGDYRIICDLQDSRLVVLVIE
IGNRRDVYR

Nucleotide

• Length: 270 bp

>T197457 NZ_CP096192:4933-5241 [Enterobacter roggenkampii]
ATGCAGTTCATGGTTTACCGCAATAACAGCAACAACCGGGCTTATCCCTATCTGCTCGATGTACAGAGCGATATTATCGA
CGAACTGAATACCCGCCTGGTGATCCCGCTGTTTCTGCTGGATGAAGTGAATGGACGGCCCGCCCGCCGCCTGACCCCGG
TGCTGAATGTGGACGGTGAGGATTTTCTGGTCATGACACATGAAATGGCCAGCGTTCGCCAGTCACAGCTAGGGGAAGAG
GTGGTGAGCGTCCGGGAGCACCGGCAGGCCATCAAGAATGCGCTGGATTTTCTGCTCGACGGTTTCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8771.74 Da
• Isoelectric Point: 4.3135

>AT197457 WP_069612387.1 NZ_CP072172:73988-74215 [Rhizobium sp. AB2/73]
MSKQTAIRLPDETYERLQALATRTGRTATFYIRQAVEAHLDEMEDVYLAERAMERIKNGEDEVVSSEEFWRGMDD

Nucleotide

• Length: 228 bp

>AT197457 NZ_CP096192:4694-4933 [Enterobacter roggenkampii]
ATGCCCGTATCAGCGATGCGCCATAAAAAAACCGTCAGTGTGACAATCGAGCCTGCGCTTTTGCATCAGGCCCGCGAAGC
CGGGATTAATCTGTCTGCCACGCTCACCACCGCCCTGAAACATCAGATCCGCGAGTCAGAAGCGGAGCGCTGGAAACGCG
CCAACCGCAAGGCAATCCAGGAGCTCAACCGTATTACGGACGAGCACGGCTTACTCTCTGACGATCACAGGACGTTCTGA

Structures

Antitoxin

• AlphaFold DB: A0A1E4YCA6