Detailed information of TA system TA197456

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RelB
• Location: 283175..283773
• Replicon: chromosome
• Accession: NZ_CP072168
• Organism: Agrobacterium larrymoorei strain AF3.44

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: J5285_RS15550
• Protein ID: WP_084631769.1
• Coordinates: 283175..283468 (-)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A4D7DQ02
• Locus tag: J5285_RS15555
• Protein ID: WP_027676543.1
• Coordinates: 283465..283773 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J5285_RS15520 (J5285_15530)	278590..279135	+	546	WP_027676548.1	sigma-70 family RNA polymerase sigma factor	-
J5285_RS15525 (J5285_15535)	279132..279836	+	705	WP_027676547.1	anti-sigma factor	-
J5285_RS15530 (J5285_15540)	279904..280722	-	819	WP_027676546.1	alpha/beta hydrolase	-
J5285_RS15535 (J5285_15545)	281233..282453	-	1221	WP_027676545.1	M20/M25/M40 family metallo-hydrolase	-
J5285_RS15540 (J5285_15550)	282500..282820	-	321	Protein_259	ABC transporter ATP-binding protein	-
J5285_RS15545 (J5285_15555)	282866..283105	+	240	WP_027676544.1	UTRA domain-containing protein	-
J5285_RS15550 (J5285_15560)	283175..283468	-	294	WP_084631769.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	Toxin
J5285_RS15555 (J5285_15565)	283465..283773	-	309	WP_027676543.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
J5285_RS15560 (J5285_15570)	284092..284911	+	820	Protein_263	manganese catalase family protein	-
J5285_RS15565 (J5285_15575)	285248..286417	-	1170	WP_027676542.1	zinc-dependent alcohol dehydrogenase	-
J5285_RS15570 (J5285_15580)	286652..287536	+	885	WP_027676541.1	PAS domain S-box protein	-
J5285_RS15575 (J5285_15585)	287721..288260	-	540	WP_027676540.1	hypothetical protein	-
J5285_RS26185	288399..288536	+	138	WP_234881830.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (4-57)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 10721.37 Da
• Isoelectric Point: 5.8086

>T197456 WP_084631769.1 NZ_CP072168:c283468-283175 [Agrobacterium larrymoorei]
MRLTWSAFALSDRDTIFTFIELENAAAAILVDERIVVAVRRLIDFPASGRAGRIAGTRELVISGTPYVAVYTITEATVRV
LRILHGAQEWPEAFSIS

Nucleotide

• Length: 294 bp

>T197456 NZ_CP096189:c75188-74703 [Enterobacter roggenkampii]
GTGGGACGTGTAACAGCACCAGAACCATTATCCAGCTCCCATCAACTAGCAGAGTTCGTCAGTGGTGAGGCAGTACTTGA
TGACTGGCTTAAACAGAGGGGATTAAAAAACCAAGCTCTTGGTGCAGCACGAACGTTCGTCGTTTGTAAAAGAGACACTA
AACAGGTTGTCGGTTTTTACTCTCTGGCCACCGGCAGCGTTAACCACGTGGAAGCAATGGGAAGCCTCAGGCGCAATATG
CCAGATCCAATTCCCGTAATCATACTTGCTCGCCTGGCAGTTGATGTTTCATACCGTGGCCAAGGGCTCGGGGCCGATTT
ACTGCATGATGCAGTTCTGCGCTGTTACCGTGTAGCTGAGAATATTGGAGTCCGTGCGATTATGGTTCATGCACTGACTG
AAGAAGCCAAAAACTTTTATCTTCACCATGGCTTTAAGGCATCACAAACTCAGGAACGGACATTGTTTTTAAAGTTACCA
CAGTGA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11185.53 Da
• Isoelectric Point: 4.8728

>AT197456 WP_027676543.1 NZ_CP072168:c283773-283465 [Agrobacterium larrymoorei]
MAANALVQTRIDADVRDRASLVLEGMGLTVSDAVRILLTRTANEGMLPLELLSGSEAHDTWFRAKVLEALNDTRPDISDD
EVEAHFEKRRHSARNNAAGITS

Nucleotide

• Length: 309 bp

>AT197456 NZ_CP096189:c75442-75176 [Enterobacter roggenkampii]
ATGAAATCAGATGTTCAGCTCAACCTTAGAGCTAAAGAATCTCAAAGAGCACTTATTGATGCTGCAGCAGAGATTCTCCA
TAAATCACGCACGGACTTCATCCTGGAAATGGCATGTCAGGCTGCGGAGAATGTGATCCTCGATCGTCGCGTATTTAATT
TTAACGATGAGCAGTATGCAGAGTTTATCGATATGCTCGATGCACCGGTTTCGGATGAGGCGGCTATTGATAAACTTCTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Structures

Antitoxin

• AlphaFold DB: A0A4D7DQ02