Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-StbC
Location 238645..239366 Replicon chromosome
Accession NZ_CP072168
Organism Agrobacterium larrymoorei strain AF3.44

Toxin (Protein)


Gene name vapC Uniprot ID A0A2Z2Q4M6
Locus tag J5285_RS15375 Protein ID WP_051441368.1
Coordinates 238645..239115 (-) Length 157 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A4D7DUK8
Locus tag J5285_RS15380 Protein ID WP_027676571.1
Coordinates 239112..239366 (-) Length 85 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J5285_RS15360 (J5285_15370) 233729..234157 - 429 WP_235695857.1 response regulator -
J5285_RS15365 (J5285_15375) 234334..236148 + 1815 WP_027676573.1 PAS domain-containing methyl-accepting chemotaxis protein -
J5285_RS15370 (J5285_15380) 236406..238595 + 2190 WP_027676572.1 EAL domain-containing protein -
J5285_RS15375 (J5285_15385) 238645..239115 - 471 WP_051441368.1 type II toxin-antitoxin system VapC family toxin Toxin
J5285_RS15380 (J5285_15390) 239112..239366 - 255 WP_027676571.1 plasmid stabilization protein Antitoxin
J5285_RS15385 (J5285_15395) 239551..240525 - 975 WP_027676570.1 class 1b ribonucleoside-diphosphate reductase subunit beta -
J5285_RS15390 (J5285_15400) 240552..242705 - 2154 WP_027676569.1 class 1b ribonucleoside-diphosphate reductase subunit alpha -
J5285_RS15395 (J5285_15405) 242675..243088 - 414 WP_027676568.1 class Ib ribonucleoside-diphosphate reductase assembly flavoprotein NrdI -
J5285_RS15400 (J5285_15410) 243098..243322 - 225 WP_027676567.1 glutaredoxin-like protein NrdH -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 222389..239366 16977


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-124)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17112.76 Da        Isoelectric Point: 7.3185

>T197455 WP_051441368.1 NZ_CP072168:c239115-238645 [Agrobacterium larrymoorei]
MILIDTNVISEPLKPAPYAAVLTWLDAQAIETLFISAISIAERRFGIASMPRGKRQMILHGRLEDEVLPIFEKRILPFNV
GTSQFYANLMASGRASGKVIGKADRYTAATAAEHDLAVATRDASPFEAAGLRVINPWATWTFKITGLEFEPHQSVG

Download         Length: 471 bp

>T197455 NZ_CP096189:23747-24034 [Enterobacter roggenkampii]
ATGGCATATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 85 a.a.        Molecular weight: 9284.60 Da        Isoelectric Point: 8.7216

>AT197455 WP_027676571.1 NZ_CP072168:c239366-239112 [Agrobacterium larrymoorei]
MAAVTIRKLSDETHRALRARATYHGRSTEAEIRDILEAAVRPSGRVKLGSLLAAIGHEAKMSKSDVEALQQQRDLTPEVP
MTFE

Download         Length: 255 bp

>AT197455 NZ_CP096189:23508-23747 [Enterobacter roggenkampii]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2Z2Q4M6

Antitoxin

Source ID Structure
AlphaFold DB A0A4D7DUK8

References