Detailed information of TA system TA197450

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 1830489..1831075
• Replicon: chromosome
• Accession: NZ_CP072167
• Organism: Agrobacterium larrymoorei strain AF3.44

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A4D7DRV4
• Locus tag: J5285_RS08625
• Protein ID: WP_027677181.1
• Coordinates: 1830815..1831075 (-)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: J5285_RS08620
• Protein ID: WP_027677182.1
• Coordinates: 1830489..1830818 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J5285_RS08600 (J5285_08605)	1826833..1827231	+	399	WP_027677339.1	DUF930 domain-containing protein	-
J5285_RS08605 (J5285_08610)	1827412..1828425	-	1014	WP_136954340.1	IS5 family transposase	-
J5285_RS08610 (J5285_08615)	1828789..1829709	+	921	WP_027677184.1	dihydrodipicolinate synthase family protein	-
J5285_RS08615 (J5285_08620)	1829764..1830486	+	723	WP_027677183.1	polysaccharide deacetylase family protein	-
J5285_RS08620 (J5285_08625)	1830489..1830818	-	330	WP_027677182.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
J5285_RS08625 (J5285_08630)	1830815..1831075	-	261	WP_027677181.1	type II toxin-antitoxin system HicA family toxin	Toxin
J5285_RS08630 (J5285_08635)	1831182..1832300	-	1119	WP_051441439.1	AI-2E family transporter	-
J5285_RS08635 (J5285_08640)	1832450..1833310	-	861	WP_027677179.1	VOC family protein	-
J5285_RS08640 (J5285_08645)	1833541..1833951	+	411	WP_027677178.1	universal stress protein	-
J5285_RS08645 (J5285_08650)	1834069..1834740	+	672	WP_027677177.1	hemolysin III family protein	-
J5285_RS08650 (J5285_08655)	1834825..1835325	+	501	WP_027677176.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1827412..1828425	1013

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (25-84)

Antitoxin

• Uncharacterised protein family HicB (54-104)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 9511.90 Da
• Isoelectric Point: 10.4752

>T197450 WP_027677181.1 NZ_CP072167:c1831075-1830815 [Agrobacterium larrymoorei]
MKLSKKHRSVLDAVFTDPALAEIDWRDIEALFQACGAEISEGSGSRVRVALNGVRAVFHRPHPQKETDKGAVKSVRRFLS
EAGIRP

Nucleotide

• Length: 261 bp

>T197450 NZ_CP096187:c1077685-1077467 [Enterobacter roggenkampii]
ATGTCTGATAAACCACTCACAAAAATCGATTATTTGATGCGCTTACGACGTTGTCAGTCAATTGACACCCTCGAACGCGT
GATTGAAAAGAATAAATACGAGCTTTCTGATAATGAACTGGCCGTATTTTATTCAGCCGCTGACCATCGTCTTGCTGAGC
TGACCATGAATAAGTTGTATGACAAAATCCCTACTTCTGTATGGAAATTTGTCCGTTAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 12265.82 Da
• Isoelectric Point: 4.7809

>AT197450 WP_027677182.1 NZ_CP072167:c1830818-1830489 [Agrobacterium larrymoorei]
MTLMHYKGYEAVIEFDEDAEIFHGEVINLRDVITFQGTSAAELKQALAESVEDYISFCKERGEEPEKPFSGQFVVRAEPR
LHKAISTAARRDGVSLNKWVTSTLEKAVS

Nucleotide

• Length: 330 bp

>AT197450 NZ_CP096187:c1078087-1077713 [Enterobacter roggenkampii]
ATGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAACCTTGATGAAAGCAACCATGGCTGGGTAAACGACCCAACGTCTGCCATCAATTTACAGTTGAATGAGCTGATAGAGC
ATATCGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGCTCATTGAGCAAATTGATGAATACCTG
GACGACACCTTTATGTTGTTCAGTAGTTACGGCATTAACGCCAAGGATTTGCAAAAATGGCGTAAATCGGGAAACCGGCT
ATTCCGCTGTTTCGTCAACGTGAGCAGAGCTAACCCGGTTAGCCTTTCCTGTTAA

Structures

Toxin

• AlphaFold DB: A0A4D7DRV4