Detailed information of TA system TA197449

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/ParE-RHH
• Location: 1784228..1784766
• Replicon: chromosome
• Accession: NZ_CP072167
• Organism: Agrobacterium larrymoorei strain AF3.44

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A4D7DQ75
• Locus tag: J5285_RS08340
• Protein ID: WP_027675635.1
• Coordinates: 1784228..1784521 (-)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A4D7DS04
• Locus tag: J5285_RS08345
• Protein ID: WP_027675634.1
• Coordinates: 1784518..1784766 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J5285_RS08320 (J5285_08325)	1780830..1781771	+	942	WP_027675639.1	prolyl oligopeptidase family serine peptidase	-
J5285_RS08325 (J5285_08330)	1781885..1782769	+	885	WP_027675638.1	L,D-transpeptidase	-
J5285_RS08330 (J5285_08335)	1782970..1783704	+	735	WP_027675637.1	L,D-transpeptidase	-
J5285_RS08335 (J5285_08340)	1783836..1784174	+	339	WP_051441286.1	NINE protein	-
J5285_RS08340 (J5285_08345)	1784228..1784521	-	294	WP_027675635.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
J5285_RS08345 (J5285_08350)	1784518..1784766	-	249	WP_027675634.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
J5285_RS08350 (J5285_08355)	1784929..1785390	-	462	WP_027675633.1	MarR family transcriptional regulator	-
J5285_RS08355 (J5285_08360)	1785585..1786010	+	426	WP_027675632.1	organic hydroperoxide resistance protein	-
J5285_RS08360 (J5285_08365)	1786134..1786517	+	384	WP_027675631.1	MbcA/ParS/Xre antitoxin family protein	-
J5285_RS08365 (J5285_08370)	1786477..1787241	+	765	WP_027675630.1	RES family NAD+ phosphorylase	-
J5285_RS08370 (J5285_08375)	1787384..1787830	-	447	WP_027675629.1	MarR family winged helix-turn-helix transcriptional regulator	-
J5285_RS08375 (J5285_08380)	1787951..1788679	+	729	WP_027675628.1	thioredoxin family protein	-
J5285_RS08380 (J5285_08385)	1788693..1789181	+	489	WP_027675627.1	DUF1579 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-90)

Antitoxin

• Antitoxin ParD (1-81)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11088.47 Da
• Isoelectric Point: 6.3222

>T197449 WP_027675635.1 NZ_CP072167:c1784521-1784228 [Agrobacterium larrymoorei]
MKALALSPAAEADLDHIWEHSATNWGLDQADRYTDEIRDACLDLAAGVRHGRPVDVRSGYLKYNTGAHMIYFRDQGDRLD
IVRILHQRQDVIFNLPK

Nucleotide

• Length: 294 bp

>T197449 NZ_CP096187:614071-614358 [Enterobacter roggenkampii]
ATGCCAACGGAGTATGAGTGGGATAGCAATAAGGCGAAAAGTAACCTGCAAAAACATGGCATACGCTTTGAGGATGCGGT
TATGGTTTTCGACGATCCTTATCATTTGTCTGTTCAGGATCGCTATGAAAACGGTGAATTTCGCTGGCAAACCATCGGCC
TTGTGCAAGGCCTTCTTGTTATTTTGGTCGCACATACGGTACGTTTTGAAAGCGGAGGAGAAATCATCCGTATCATTAGC
GCACGAAAGGCGGACCGCAAAGAGAGGAGCCGTTATGAGCATCGTTAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9218.25 Da
• Isoelectric Point: 7.2036

>AT197449 WP_027675634.1 NZ_CP072167:c1784766-1784518 [Agrobacterium larrymoorei]
MARNTSITLGDHFASFIRDQVQTGRYGSTSDVVRAGLRLLEEHEAKVKSLQDALIAGEQSGEPRAFDFEAFKTRKRAEYE
GR

Nucleotide

• Length: 249 bp

>AT197449 NZ_CP096187:614345-614647 [Enterobacter roggenkampii]
ATGAGCATCGTTAAACATAAACGCGGTAGTGCGCCTGAGGTAAGCGCTCGTCGTGTGGCTGAGCTCAAAGCATTAGCTGA
AAAACCAGATGCGGAGATTGATTACAGCGATATTCCTGCCACGGACGATGAACAGTGGTCAGGGGCGGTGCGAGGGAAGT
TCTATCGACCACTCAAAACTCAGGCATCTGTACGCATTGACGCCGATGTGATGGAGTGGCTTAAACGGCCTGGGAAAGGT
TATCAGACGCGGCTCAATGCCATTTTGCGTGAAGCAATGCTGCGCGATCAAAACAAAAAATAA

Structures

Toxin

• AlphaFold DB: A0A4D7DQ75

Antitoxin

• AlphaFold DB: A0A4D7DS04